Recombinant Anti-Glutaminase antibody [EP7212] (ab156876)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP7212] to Glutaminase
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Glutaminase antibody [EP7212]
See all Glutaminase primary antibodies -
Description
Rabbit monoclonal [EP7212] to Glutaminase -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human kidney tissue lysates, HeLa and 293T cell lysates, wild type HAP1 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP7212 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 73 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 73 kDa. |
Target
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Function
Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. -
Tissue specificity
KGA is expressed predominantly in brain and kidney but not in liver, isoform 3 is expressed principally in cardiac muscle and pancreas but not in liver or brain, and isoform 2 is expressed solely in cardiac and skeletal muscle. -
Sequence similarities
Belongs to the glutaminase family.
Contains 1 ANK repeat. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 2744 Human
- Omim: 138280 Human
- SwissProt: O94925 Human
- Unigene: 116448 Human
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Alternative names
- AAD20 antibody
- DKFZp686O15119 antibody
- FLJ10358 antibody
see all
Images
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All lanes : Anti-Glutaminase antibody [EP7212] (ab156876) at 1/1000 dilution
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 73 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-Glutaminase antibody [EP7212] (ab156876) at 1/1000 dilution
Lane 1 : Human kidney tissue lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 73 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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Lane 1: Wild type HAP1 whole cell lysate (20 µg) Lane 2: GLS knockout HAP1 whole cell lysate (20 µg) Lane 3: HeLa whole cell lysate (20 µg) Lane 4: HEK293 whole cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab156876 observed at 62 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab156876 was shown to recognize GLS in wild-type HAP1 cells as signal was lost at the expected MW in HAP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GLS knockout samples were subjected to SDS-PAGE. Ab156876 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.
Blue arrow corresponds to isoform 1 KGA.
Red arrow corresponds to isoform 3 GAC.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (77)
ab156876 has been referenced in 77 publications.
- Chen W et al. Qici Sanling Decoction Suppresses Glutamine Consumption and Bladder Cancer Cell Growth through Inhibiting c-Myc Expression. J Oncol 2022:7985468 (2022). PubMed: 35058981
- Wang L et al. Spatially resolved isotope tracing reveals tissue metabolic activity. Nat Methods 19:223-230 (2022). PubMed: 35132243
- Huynh VN et al. Acute inhibition of OGA sex-dependently alters the networks associated with bioenergetics, autophagy, and neurodegeneration. Mol Brain 15:22 (2022). PubMed: 35248135
- Farah H et al. Differential Metabotypes in Synovial Fibroblasts and Synovial Fluid in Hip Osteoarthritis Patients Support Inflammatory Responses. Int J Mol Sci 23:N/A (2022). PubMed: 35328687
- Mohás A et al. In Situ Analysis of mTORC1/C2 and Metabolism-Related Proteins in Pediatric Osteosarcoma. Pathol Oncol Res 28:1610231 (2022). PubMed: 35392503