Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP7212] to Glutaminase (HRP)
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Product nameAnti-Glutaminase antibody [EP7212] (HRP)
See all Glutaminase primary antibodies
DescriptionRabbit monoclonal [EP7212] to Glutaminase (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human Glutaminase aa 50-150. The exact sequence is proprietary.
- WB: Human fetal kidney lysate.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-Glutaminase antibody [EP7212] (ab156876)
- Anti-Glutaminase antibody [EP7212] (Alexa Fluor® 488) (ab200406)
- Anti-Glutaminase antibody [EP7212] (Alexa Fluor® 647) (ab200407)
- Anti-Glutaminase antibody [EP7212] (Alexa Fluor® 555) (ab207272)
- Anti-Glutaminase antibody [EP7212] - BSA and Azide free (ab214802)
Our Abpromise guarantee covers the use of ab200408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).|
FunctionCatalyzes the first reaction in the primary pathway for the renal catabolism of glutamine.
Tissue specificityKGA is expressed predominantly in brain and kidney but not in liver, isoform 3 is expressed principally in cardiac muscle and pancreas but not in liver or brain, and isoform 2 is expressed solely in cardiac and skeletal muscle.
Sequence similaritiesBelongs to the glutaminase family.
Contains 1 ANK repeat.
- Information by UniProt
- AAD20 antibody
- DKFZp686O15119 antibody
- FLJ10358 antibody
All lanes : Anti-Glutaminase antibody [EP7212] (HRP) (ab200408) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : GLS (Glutaminase) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 73 kDa
Exposure time: 12 minutes
ab200408 was shown to recognize Glutaminase in wild-type HAP1 cells as signal was lost at the expected MW in GLS (Glutaminase) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GLS (Glutaminase) knockout samples were subjected to SDS-PAGE. Ab200408 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
Anti-Glutaminase antibody [EP7212] (HRP) (ab200408) at 1/5000 dilution + Kidney (Human) Tissue Lysate - fetal normal tissue at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 73 kDa
Observed band size: 73 kDa
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab200408 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab200408 has been referenced in 1 publication.
- Karaca M et al. Liver Glutamate Dehydrogenase Controls Whole Body Energy Partitioning Through Amino Acid-Derived Gluconeogenesis and Ammonia Homeostasis. Diabetes N/A:N/A (2018). PubMed: 30002133