Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Glutaminase antibody [EP7212] (HRP) (ab200408)

Overview

  • Product name

    Anti-Glutaminase antibody [EP7212] (HRP)
    See all Glutaminase primary antibodies
  • Description

    Rabbit monoclonal [EP7212] to Glutaminase (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Glutaminase aa 50-150. The exact sequence is proprietary.

  • Positive control

    • WB: Human fetal kidney lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab200408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).

Target

  • Function

    Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine.
  • Tissue specificity

    KGA is expressed predominantly in brain and kidney but not in liver, isoform 3 is expressed principally in cardiac muscle and pancreas but not in liver or brain, and isoform 2 is expressed solely in cardiac and skeletal muscle.
  • Sequence similarities

    Belongs to the glutaminase family.
    Contains 1 ANK repeat.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • AAD20 antibody
    • DKFZp686O15119 antibody
    • FLJ10358 antibody
    • GAC antibody
    • GAM antibody
    • GLS antibody
    • GLS1 antibody
    • GLSK_HUMAN antibody
    • Glutaminase C antibody
    • Glutaminase kidney isoform antibody
    • Glutaminase phosphate activated antibody
    • K-glutaminase antibody
    • KGA antibody
    • KIAA0838 antibody
    • L glutamine amidohydrolase antibody
    • L-glutamine amidohydrolase antibody
    • mitochondrial antibody
    see all

Images

  • All lanes : Anti-Glutaminase antibody [EP7212] (HRP) (ab200408) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : GLS (Glutaminase) knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 73 kDa


    Exposure time: 12 minutes


    ab200408 was shown to recognize Glutaminase in wild-type HAP1 cells as signal was lost at the expected MW in GLS (Glutaminase) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GLS (Glutaminase) knockout samples were subjected to SDS-PAGE. Ab200408 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

  • Anti-Glutaminase antibody [EP7212] (HRP) (ab200408) at 1/5000 dilution + Kidney (Human) Tissue Lysate - fetal normal tissue at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 73 kDa
    Observed band size: 73 kDa


    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab200408 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

This product has been referenced in:

  • Karaca M  et al. Liver Glutamate Dehydrogenase Controls Whole Body Energy Partitioning Through Amino Acid-Derived Gluconeogenesis and Ammonia Homeostasis. Diabetes N/A:N/A (2018). Read more (PubMed: 30002133) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (kidney)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Specification
kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 14 2017

Application
Western blot
Sample
Mouse Cell lysate - whole cell (hepatoma cell line)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
35 µg
Specification
hepatoma cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 09 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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