Product nameAnti-Glutaminase C antibody
See all Glutaminase C primary antibodies
DescriptionRabbit polyclonal to Glutaminase C
Tested applicationsSuitable for: IHC-P, WB, ICC/IF, IPmore details
Species reactivityReacts with: Human
Recombinant fragment within Human Glutaminase C (C terminal). The exact sequence is proprietary.
Database link: O94925
- ICC/IF: A549 cells. IHC-P: Human breast carcinoma tissue. WB: GLS1(GAC isoform specific) shRNA-transfected MDA-MB-231 whole cell extract. IP: Glutaminase C protein from MDA-MB-231 whole cell extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab262717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 73 kDa.|
|ICC/IF||1/100 - 1/1000.|
|IP||1/100 - 1/500.|
RelevanceCatalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity. Tissue specificityi Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle
- SwissProt: O94925-3 Human
All lanes : Anti-Glutaminase C antibody (ab262717) at 1/5000 dilution
Lane 1 : Non-transfected MDA-MB-231 whole cell extract
Lane 2 : GLS1(GAC isoform specific) shRNA-transfected MDA-MB-231 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 73 kDa
This antibody specifically recognizes GAC but not the KGA isoform.
Paraffin embedded human breast carcinoma tissue stained for Glutaminase C using ab262717 at a 1/500 dilution in immunohistochemistry.
Immunoprecipitation of Glutaminase C protein from MDA-MB-231 whole cell extracts using 5 μg of ab262717.
Western blot analysis was performed using ab262717.
EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Input
Lane 2: Control IgG IP
Lane 3: ab262717 IP
A549 cells stained for Glutaminase C using ab262717 at a 1/500 dilution (green) in ICC/IF.
Cells were fixed in 4% paraformaldehyde at RT for 15 mins.
Red: Histone H3K9ac (acetyl Lys9), a nucleus marker, stained by Histone H3K9ac (acetyl Lys9) antibody diluted at 1/500.
Scale bar = 10 μm.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab262717 has not yet been referenced specifically in any publications.