Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 90 min
- Sample type: Other biological fluids, Plasma, Serum, Tissue, Urine
- Sensitivity: 25 µM
Product nameGlutamine Assay Kit (Colorimetric)
Sample typeUrine, Serum, Plasma, Other biological fluids, Tissue
Assay time0h 90m
Species reactivityReacts with: Mammals, Other species
Glutamine Assay kit (Colorimetric) ab197011 is a simple and sensitive assay that detects the biologically relevant concentrations of glutamine in biological fluids and tissues.
The glutamine assay protocol is based on the hydrolysis of Glutamine to Glutamate producing a stable colored signal, which is directly proportional to the amount of glutamine in the sample.
The assay can detect as little as 25 μM of glutamine in a variety of biological samples.
Glutamine assay protocol summary:
- add samples and standards to wells
- add hydrolysis mix and incubate for 30 min
- add reaction mix and incubate for 60 min
- analyze with a microplate reader.
Previously called Glutamine Detection Assay Kit (Colorimetric).
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Developer 1 vial Development Buffer 1 x 25ml Development Enzyme Mix (lyophilized) Green 1 vial Glutamine Standard 1 vial Hydrolysis Buffer NM 1 x 25ml Hydrolysis Enzyme Mix 1 vial
RelevanceGlutamine (abbreviated as Gln or Q; the abbreviation Glx or Z represents either glutamine or glutamic acid) is one of the 20 amino acids encoded by the standard genetic code. Its side chain is an amide formed by replacing the side-chain hydroxyl of glutamic acid with an amine functional group. It can therefore be considered the amide of glutamic acid. Its codons are CAA and CAG. Recent studies have proven its effectiveness in anabolic muscle growth from prolonged consumption.
Glutamine Standard Curve. For demonstrative purposes only.
Measurement of Glutamine concentration in Human urine (5 μl).
Measurement of Glutamine concentration in Mouse tissues (brain [15 μg], liver [50 μg]). Samples were deproteinized using 10K Spin Column and spiked with known amount of Glutamine (6 nmol).
ab197011 has been referenced in 9 publications.
- Morotti M et al. Increased expression of glutamine transporter SNAT2/SLC38A2 promotes glutamine dependence and oxidative stress resistance, and is associated with worse prognosis in triple-negative breast cancer. Br J Cancer 124:494-505 (2021). PubMed: 33028955
- Edwards DN et al. Selective glutamine metabolism inhibition in tumor cells improves antitumor T lymphocyte activity in triple-negative breast cancer. J Clin Invest 131:N/A (2021). PubMed: 33320840
- Ito J et al. Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice. Elife 10:N/A (2021). PubMed: 33526170
- Chen P et al. Targeting YTHDF1 effectively re-sensitizes cisplatin-resistant colon cancer cells by modulating GLS-mediated glutamine metabolism. Mol Ther Oncolytics 20:228-239 (2021). PubMed: 33614908
- Birkner K et al. ß1-Integrin- and KV1.3 channel-dependent signaling stimulates glutamate release from Th17 cells. J Clin Invest 130:715-732 (2020). PubMed: 31661467
- Zhang L et al. PK-M2-mediated metabolic changes in breast cancer cells induced by ionizing radiation. Breast Cancer Res Treat 178:75-86 (2019). PubMed: 31372790
- Chen Y et al. miR-206 inhibits osteogenic differentiation of bone marrow mesenchymal stem cells by targetting glutaminase. Biosci Rep 39:N/A (2019). PubMed: 30804229
- Zhang K et al. miR-9 regulates ferroptosis by targeting glutamic-oxaloacetic transaminase GOT1 in melanoma. Mol Carcinog N/A:N/A (2018). PubMed: 30035324
- Li DD et al. Inhibition of the oxidative stress-induced miR-23a protects the human retinal pigment epithelium (RPE) cells from apoptosis through the upregulation of glutaminase and glutamine uptake. Mol Biol Rep 43:1079-87 (2016). PubMed: 27411920