Overview

  • Product name

    Glutamine Assay Kit (Colorimetric)
  • Detection method

    Colorimetric
  • Sample type

    Urine, Serum, Plasma, Other biological fluids, Tissue
  • Assay type

    Quantitative
  • Sensitivity

    25 µM
  • Assay time

    0h 90m
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Glutamine Assay kit (Colorimetric) ab197011 is a simple and sensitive assay that detects the biologically relevant concentrations of glutamine in biological fluids and tissues.


    The glutamine assay protocol is based on the hydrolysis of Glutamine to Glutamate producing a stable colored signal (OD 450 nm), which is directly proportional to the amount of glutamine in the sample.


    The assay can detect as little as 25 μM of glutamine in a variety of biological samples.


    Glutamine assay protocol summary:
    - add samples and standards to wells
    - add hydrolysis mix and incubate for 30 min
    - add reaction mix and incubate for 60 min
    - analyze with a microplate reader.

  • Notes

    Previously called Glutamine Detection Assay Kit (Colorimetric).

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Developer 1 vial
    Development Buffer 1 x 25ml
    Development Enzyme Mix (lyophilized) Green 1 vial
    Glutamine Standard 1 vial
    Hydrolysis Buffer NM 1 x 25ml
    Hydrolysis Enzyme Mix 1 vial
  • Research areas

  • Relevance

    Glutamine (abbreviated as Gln or Q; the abbreviation Glx or Z represents either glutamine or glutamic acid) is one of the 20 amino acids encoded by the standard genetic code. Its side chain is an amide formed by replacing the side-chain hydroxyl of glutamic acid with an amine functional group. It can therefore be considered the amide of glutamic acid. Its codons are CAA and CAG. Recent studies have proven its effectiveness in anabolic muscle growth from prolonged consumption.
  • Alternative names

    • Gln
    • Q

Images

  • Measurement of Glutamine concentration in Human urine (5 μl).

  • Measurement of Glutamine concentration in Mouse tissues (brain [15 μg], liver [50 μg]). Samples were deproteinized using 10K Spin Column and spiked with known amount of Glutamine (6 nmol).

Protocols

References

This product has been referenced in:

  • Zhang L  et al. PK-M2-mediated metabolic changes in breast cancer cells induced by ionizing radiation. Breast Cancer Res Treat 178:75-86 (2019). Read more (PubMed: 31372790) »
  • Zhang K  et al. miR-9 regulates ferroptosis by targeting glutamic-oxaloacetic transaminase GOT1 in melanoma. Mol Carcinog N/A:N/A (2018). Read more (PubMed: 30035324) »
See all 3 Publications for this product

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