Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (Drosophila S2 cell)
Loading amount
20 µg
Specification
Drosophila S2 cell
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/3000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C
Secondary antibody
Name
Non-Abcam antibody was used: alpha-mouse IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 43 kDa
Additional data
Additional Notes
lysis buffer : RIPA lysis buffer
Drosophila S2 cell whole cell lysate
Size marker : SeeBlue plus 2 (Invitrogen)
Membrane : ECL hybond+
Drosophila S2 cell whole cell lysate
Size marker : SeeBlue plus 2 (Invitrogen)
Membrane : ECL hybond+
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Myoung Sup Shim
Verified customer
Submitted Apr 14 2009