Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Zebrafish Tissue sections (Retina, Muller glia cells)
Specification
Retina, Muller glia cells
Permeabilization
Yes - 0.1% TritonX
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Block solution
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Alexa 647 conjugated goat anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Dilution
1/1000
Additional data
Additional Notes
No labelling in the retina at 4 days post fertilization
Abcam response
Thank you for submitting an Abreview. ab64613 does not seem to work under these experimental conditions. Thank you for your time, this information will surely be helpful for customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ryan Macdonald
Verified customer
Submitted May 28 2013