Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Nothobranchius furzeri Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
Yes - Triton
Specification
Brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 21°C
Fixative
Formaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 21°C
Dilution
1/900
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Jolien Van houcke
Verified customer
Submitted Oct 07 2017