Application
Immunohistochemistry (Frozen sections)
Sample
Zebrafish Tissue sections (retina)
Permeabilization
Yes - PBS/0.05% Trition X100 (PBS-TX) for 10 min
Specification
retina
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Diluent: 1X Casein in PBS/0.05% Trition X-100
Dilution
1/1000
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Donkey anti-Mouse IgG (H+L) Alexa fluor 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
Outer segments shows only autofluorescence.
Abcam response
Thank you for your time and valuable feedback. We have not previously tested this antibody in frozen sections and it does not seem to work under these experimental conditions.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
ANN FROYSET
Verified customer
Submitted Jun 28 2021