Overview

  • Product name
    Anti-Glutamine Synthetase antibody
    See all Glutamine Synthetase primary antibodies
  • Description
    Rabbit polyclonal to Glutamine Synthetase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, WB, In-Cell ELISA, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Cow, Dog, Chimpanzee, Ferret, Zebrafish
  • Immunogen

    Recombinant full length protein purified from E.coli (Human).

  • Positive control
    • Mouse and Rat brain extract. Mouse liver extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab16802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-Fr Use at an assay dependent concentration.
WB 1/2000. Predicted molecular weight: 42 kDa.
In-Cell ELISA Use at an assay dependent concentration.
IHC-FoFr 1/100.

Target

  • Function
    This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
  • Involvement in disease
    Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
  • Sequence similarities
    Belongs to the glutamine synthetase family.
  • Developmental stage
    Expressed during early fetal stages.
  • Cellular localization
    Cytoplasm. Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • cell proliferation-inducing protein 59 antibody
    • GLNA antibody
    • GLNA_HUMAN antibody
    • GLNS antibody
    • GLUL antibody
    • Glutamate ammonia ligase antibody
    • Glutamate decarboxylase antibody
    • Glutamate--ammonia ligase antibody
    • glutamine synthase antibody
    • Glutamine synthetase antibody
    • GS antibody
    • PIG 43 antibody
    • PIG 59 antibody
    • PIG43 antibody
    • PIG59 antibody
    • Proliferation inducing protein 43 antibody
    see all

Images

  • ab16802 at a 1/2000 dilution staining approx. 42kDa Glutamine synthetase in 1) 293T cell lysate transfected with Myc-Glutamine synthetase, 2) mouse brain lysate, 3) mouse liver lysate and 4) rat brain lysate by Western blot (ECL). ab16802 at a 1/2000 dilution staining approx. 42kDa Glutamine synthetase in 1) 293T cell lysate transfected with Myc-Glutamine synthetase, 2) mouse brain lysate, 3) mouse liver lysate and 4) rat brain lysate by Western blot (ECL).
  • Left panel shows scanned image of an amine coated plate seeded with a titration of HeLa cells and stained with different dilutions of ab16082 using In Cell ELISA support pack ab111542 and protocol. Right panel shows bar graph of normalized signal intensity in 3 different cells lines after staining with ab160802 at 1/2500 dilution. Average intra-assay coefficient of variation was calculated at 5.6% and Z score at 0.77.

  • HeLa cells were seeded on glass coverslips and allow to adhere overnight. Cells were fixed with 4% paraformaldehyde for 15 min and washed in PBS x3. ICC procedure was carried out using In Cell ELISA support pack ab111541 and protocol. Left panel shows HeLa cells stained with ab16802 at a dilution of 1/1000, labeled with Goat anti-rabbit 594 secondary antibody and counterstain with DAPI. Right panel shows co-localization of the signal with the mitochondrial marker Anti-ATPB antibody [3D5] ab14730 (4 �g/mL) labeled with Goat anti-mouse 488 secondary antibody.
  • ICC/IF image of ab16802 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16802, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Tietze L  et al. Assessment of the hepatocytic differentiation ability of human skin-derived ABCB5+ stem cells. Exp Cell Res 369:335-347 (2018). Read more (PubMed: 29864400) »
  • Zalis MC  et al. Immunocytochemical Profiling of Cultured Mouse Primary Retinal Cells. J Histochem Cytochem 65:223-239 (2017). Read more (PubMed: 28151698) »
See all 22 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for contacting us.
I am sorry that the antibody is not working as expected.
If you please could send me an image of your staining, that would be of great help.
I'd be happy to further discuss options of resolving this case.
I look forward to hear back from you.

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Answer

Thank you for contacting us.

We carry 13 antibodies to glutamine synthetase. None of these have so far been tested in yeast.

I have 2 questions for you:

1) What application/technique are you planning to use the antibody in?
2) Could you please provide the yeast GS sequence?

It would be worthwhile to check sequence homology with the immunogen of these antibodies as crossreactivity might be likely. I can check this for you, if you could kindly send me the yeast sequence.

If the homology is 85% or higher, you would be eligible to use our testing discount program. Here is some more information:

For UNTESTED species and/or applications, we have established a testing discount program. A brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody/protein/kit at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
This programapplies to ELISA kits and other kits we offer.

I look forward to hear back from you and assist your further.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab49873.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

Read More

Answer

Thank you for your reply.
I think you would not really see an overlap with the nucleus as GS is more located in the cytoplasm and mitochondria, but the staining should be more intense in general.
I'd be happy to replace, credit or refund the antibody. Please let me know which option would be best.
We have the following antibodies that have been tested in IHC-Fr as well as rat: ab64613, ab73593, and ab49873.
I look forward to hear back from you.

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Answer

Thank you for your email and the image.
The staining does look like non-specific staining. I have a couple questions in this regard:
1) What antibody dilution was used for this image?
2) Where would the nuclei be located in relation to the "red circles"?
3) And where would the GFP be located in relation to these circles?
4) Also, do you have any other image with brighter red staining? Or is it always this dim?
If this image is representative of the results you get, it would indicate a problem with the staining and potentially the primary antibody.
I look forward to hear back from you and assist you further.

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Answer

Thank you for your enquiry. We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased. These conditions will vary among our antibodies, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at least six month from the date of purchase when stored correctly. For this antibody when stored correctly I would in general expect a shelf life of approximately 5 years. But this can also be longer and has to be experimentally tested in any case. For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page (https://www.abcam.com/index.html?pageconfig=popular_protocols). I hope this information helps. Please do not hesitate to contact us if you need anything further.

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