Key features and details
- Goat polyclonal to Glutamine Synthetase
- Suitable for: WB, IP, Sandwich ELISA
- Isotype: IgG
Product nameAnti-Glutamine Synthetase antibody
See all Glutamine Synthetase primary antibodies
DescriptionGoat polyclonal to Glutamine Synthetase
Tested applicationsSuitable for: WB, IP, Sandwich ELISAmore details
Species reactivityReacts with: Other species
Full length native protein (purified) corresponding to Glutamine Synthetase. (Brevibacterium).
- Glutamine Synthetase (Microbial).
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride
Concentration information loading...
Purification notesab181625 is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Our Abpromise guarantee covers the use of ab181625 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 53 kDa.|
|Sandwich ELISA||1/5000 - 1/20000.
ab181625 has been assayed against 1.0 μg of Glutamine Synthetase (microbial) in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
FunctionThis enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
Involvement in diseaseDefects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
Sequence similaritiesBelongs to the glutamine synthetase family.
Developmental stageExpressed during early fetal stages.
Cellular localizationCytoplasm. Mitochondrion.
- Information by UniProt
- cell proliferation-inducing protein 59 antibody
- Cgl2214 antibody
- GLNA antibody
Anti-Glutamine Synthetase antibody (ab181625) at 1/1000 dilution + Glutamine Synthetase at 0.05 µg
Peroxidase anti-Goat at 1/40000 dilution
Predicted band size: 53 kDa
All lanes : Anti-Glutamine Synthetase antibody (ab181625) at 1/3000 dilution
Lane 1 : Glutamine Synthetase Reduced
Lane 2 : Glutamine Synthetase Non-reduced
Lysates/proteins at 1 µg per lane.
All lanes : Dylight 649 conjugated Donkey anti goat at 1/10000 dilution
Predicted band size: 53 kDa
Other band(s): Glutamine Synthetase splice variants and isoforms.
ab181625 has not yet been referenced specifically in any publications.