Overview

  • Product name
    Anti-Glutamine Synthetase antibody
    See all Glutamine Synthetase primary antibodies
  • Description
    Goat polyclonal to Glutamine Synthetase
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IP, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Other species
  • Immunogen

    Full length native protein (purified) corresponding to Glutamine Synthetase. (Brevibacterium).

  • Positive control
    • Glutamine Synthetase (Microbial).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    ab181625 is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 53 kDa.
IP 1/100.
Sandwich ELISA 1/5000 - 1/20000.

ab181625 has been assayed against 1.0 μg of Glutamine Synthetase (microbial) in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.

Target

  • Function
    This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
  • Involvement in disease
    Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
  • Sequence similarities
    Belongs to the glutamine synthetase family.
  • Developmental stage
    Expressed during early fetal stages.
  • Cellular localization
    Cytoplasm. Mitochondrion.
  • Information by UniProt
  • Alternative names
    • cell proliferation-inducing protein 59 antibody
    • GLNA antibody
    • GLNA_HUMAN antibody
    • GLNS antibody
    • GLUL antibody
    • Glutamate ammonia ligase antibody
    • Glutamate decarboxylase antibody
    • Glutamate--ammonia ligase antibody
    • glutamine synthase antibody
    • Glutamine synthetase antibody
    • GS antibody
    • PIG 43 antibody
    • PIG 59 antibody
    • PIG43 antibody
    • PIG59 antibody
    • Proliferation inducing protein 43 antibody
    see all

Images

  • Anti-Glutamine Synthetase antibody (ab181625) at 1/1000 dilution + Glutamine Synthetase at 0.05 µg

    Secondary
    Peroxidase anti-Goat at 1/40000 dilution

    Predicted band size: 53 kDa

  • All lanes : Anti-Glutamine Synthetase antibody (ab181625) at 1/3000 dilution

    Lane 1 : Glutamine Synthetase Reduced
    Lane 2 : Glutamine Synthetase Non-reduced

    Lysates/proteins at 1 µg per lane.

    Secondary
    All lanes : Dylight 649 conjugated Donkey anti goat at 1/10000 dilution

    Predicted band size: 53 kDa



    Other band(s): Glutamine Synthetase splice variants and isoforms.

References

ab181625 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181625.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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