Anti-Glutamine Synthetase antibody (ab228590)
Key features and details
- Rabbit polyclonal to Glutamine Synthetase
- Suitable for: IHC-P, WB, IP, IHC-Fr, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Glutamine Synthetase antibody
See all Glutamine Synthetase primary antibodies -
Description
Rabbit polyclonal to Glutamine Synthetase -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IP, IHC-Fr, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog, Pig, Chimpanzee, Rhesus monkey -
Immunogen
Recombinant full length protein corresponding to Human Glutamine Synthetase aa 1 to the C-terminus.
Database link: P15104 -
Positive control
- WB: U-87 MG, HEK-293T, SK-N-SH, IMR32, SK-N-AS whole cell extracts; Mouse eye and brain tissue extracts; Rat brain tissue extract. ICC/IF: DIV9 rat E18 primary cortical neurons. IHC-P: H441 xenograft tissue; Adult mouse retina tissue. IHC-Fr: Adult mouse cerebellum tissue. IP: IMR32 whole cell extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/50000. Predicted molecular weight: 42 kDa.
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IP |
1/100 - 1/500.
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IHC-Fr |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/50000. Predicted molecular weight: 42 kDa. |
IP
1/100 - 1/500. |
IHC-Fr
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts. -
Involvement in disease
Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid. -
Sequence similarities
Belongs to the glutamine synthetase family. -
Developmental stage
Expressed during early fetal stages. -
Cellular localization
Cytoplasm. Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 281199 Cow
- Entrez Gene: 2752 Human
- Entrez Gene: 14645 Mouse
- Entrez Gene: 396944 Pig
- Entrez Gene: 24957 Rat
- Omim: 138290 Human
- SwissProt: P15103 Cow
- SwissProt: Q8HZM5 Dog
see all -
Alternative names
- cell proliferation-inducing protein 59 antibody
- Cgl2214 antibody
- GLNA antibody
see all
Images
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Paraffin-embedded adult mouse retina tissue stained for Glutamine Synthetase (green) using ab228590 at 1/250 dilution in immunohistochemical analysis.
Nuclear counter stain: Fluoroshield with DAPI (blue). beta Tubulin 3/Tuj1 was stained by an anti-beta Tubulin 3/Tuj1 antibody (red) at 1/250 dilution.
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Paraffin-embedded H441 (human) xenograft tissue stained for Glutamine Synthetase using ab228590 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-Glutamine Synthetase antibody (ab228590) at 1/5000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 whole cell extract
Lane 4 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Frozen sections of adult mouse cerebellum tissue stained for Glutamine Synthetase (green) using ab228590 at 1/250 dilution in immunohistochemical analysis.
Nuclear counter stain: Fluoroshield with DAPI (blue). beta Tubulin 3/Tuj1 was stained by an anti-beta Tubulin 3/Tuj1 antibody (red) at 1/500 dilution.
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Anti-Glutamine Synthetase antibody (ab228590) at 1/20000 dilution + Mouse eye tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons stained for Glutamine Synthetase (green) using ab228590 at 1/500 dilution in ICF/IF.
Nuclear counter stain: Fluoroshield with DAPI (blue). beta Tubulin 3/Tuj1 was stained by an anti-beta Tubulin 3/Tuj1 antibody (red) at 1/500 dilution.
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Glutamine Synthetase was immunoprecipitated from IMR32 whole cell extract with 5 µg of ab228590. Western blot was performed from the immunoprecipitate using ab228590. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG instead of ab228590 in IMR32 whole cell extract.
Lane 2: ab228590 IP in IMR32 whole cell extract. -
Anti-Glutamine Synthetase antibody (ab228590) at 1/50000 dilution + Mouse brain tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Anti-Glutamine Synthetase antibody (ab228590) at 1/50000 dilution + Rat brain tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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All lanes : Anti-Glutamine Synthetase antibody (ab228590) at 1/1000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract.
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Glutamine Synthetase shRNA, whole cell extract.
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab228590 has been referenced in 4 publications.
- Li X et al. Targeting long noncoding RNA-AQP4-AS1 for the treatment of retinal neurovascular dysfunction in diabetes mellitus. EBioMedicine 77:103857 (2022). PubMed: 35172268
- Sun TT et al. Regulatory effect of long-stranded non-coding RNA-CRNDE on neurodegeneration during retinal ischemia-reperfusion. Heliyon 8:e10994 (2022). PubMed: 36276743
- Sun Y et al. Mitophagy Protects the Retina Against Anti-Vascular Endothelial Growth Factor Therapy-Driven Hypoxia via Hypoxia-Inducible Factor-1α Signaling. Front Cell Dev Biol 9:727822 (2021). PubMed: 34790659
- Madaan A et al. Müller Cell-Localized G-Protein-Coupled Receptor 81 (Hydroxycarboxylic Acid Receptor 1) Regulates Inner Retinal Vasculature via Norrin/Wnt Pathways. Am J Pathol 189:1878-1896 (2019). PubMed: 31220454