Key features and details
- Rabbit polyclonal to Glutamine Synthetase
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Glutamine Synthetase antibody
See all Glutamine Synthetase primary antibodies
DescriptionRabbit polyclonal to Glutamine Synthetase
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog
Synthetic peptide within Human Glutamine Synthetase aa 275-325. The exact sequence is proprietary.
Database link: P15104
- IP: HEK-293T whole cell lysate.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
Use at 2-10 µg/mg of lysate.
FunctionThis enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
Involvement in diseaseDefects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
Sequence similaritiesBelongs to the glutamine synthetase family.
Developmental stageExpressed during early fetal stages.
Cellular localizationCytoplasm. Mitochondrion.
- Information by UniProt
- cell proliferation-inducing protein 59 antibody
- Cgl2214 antibody
- GLNA antibody
Glutamine Synthetase was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264395 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab264395 at 0.4 µg/ml.
Lane 1: ab264395 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Lysates prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264395 has not yet been referenced specifically in any publications.