Anti-Glutamine Synthetase antibody (ab49873)
Key features and details
- Rabbit polyclonal to Glutamine Synthetase
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Glutamine Synthetase antibody
See all Glutamine Synthetase primary antibodies -
Description
Rabbit polyclonal to Glutamine Synthetase -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Chicken, Hamster, Cow, Dog, Human, Pig, Cynomolgus monkey
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Immunogen
Synthetic peptide corresponding to Mouse Glutamine Synthetase aa 357-373 (C terminal).
Sequence:RTCLLNETGDEPFQYKN
Database link: P09606 -
Positive control
- WB: Rat brain whole extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Purification notes
Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab49873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000. Predicted molecular weight: 42 kDa. |
Target
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Function
This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts. -
Involvement in disease
Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid. -
Sequence similarities
Belongs to the glutamine synthetase family. -
Developmental stage
Expressed during early fetal stages. -
Cellular localization
Cytoplasm. Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 396489 Chicken
- Entrez Gene: 281199 Cow
- Entrez Gene: 2752 Human
- Entrez Gene: 396944 Pig
- Entrez Gene: 24957 Rat
- Omim: 138290 Human
- SwissProt: P16580 Chicken
- SwissProt: P15103 Cow
see all -
Alternative names
- cell proliferation-inducing protein 59 antibody
- Cgl2214 antibody
- GLNA antibody
see all
Images
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Western blot - Anti-Glutamine Synthetase antibody (ab49873)Lane 1 : Anti-Glutamine Synthetase antibody (ab49873) at 1/5000 dilution
Lane 2 : Anti-Glutamine Synthetase antibody (ab49873) at 1/10000 dilution
Lane 3 : Anti-Glutamine Synthetase antibody (ab49873) at 1/20000 dilution
All lanes : Rat brain whole extract
Secondary
All lanes : Goat Anti-Rabbit IgG-Peroxidase
Predicted band size: 42 kDa
Protocols
References (38)
ab49873 has been referenced in 38 publications.
- García-Bonilla M et al. Neocortical tissue recovery in severe congenital obstructive hydrocephalus after intraventricular administration of bone marrow-derived mesenchymal stem cells. Stem Cell Res Ther 11:121 (2020). PubMed: 32183876
- Ma R et al. Metabolic and non-metabolic liver zonation is established non-synchronously and requires sinusoidal Wnts. Elife 9:N/A (2020). PubMed: 32154783
- Scheidecker B et al. Induction of in vitro Metabolic Zonation in Primary Hepatocytes Requires Both Near-Physiological Oxygen Concentration and Flux. Front Bioeng Biotechnol 8:524 (2020). PubMed: 32656187
- Chen Y et al. Fine-scale visualizing the hierarchical structure of mouse biliary tree with fluorescence microscopy method. Biosci Rep 40:N/A (2020). PubMed: 32364232
- Pereiro X et al. Optimization of a Method to Isolate and Culture Adult Porcine, Rats and Mice Müller Glia in Order to Study Retinal Diseases. Front Cell Neurosci 14:7 (2020). PubMed: 32082123
