Anti-Glutamine Synthetase antibody (ab49873)
- Datasheet
- Specific References (19)
- Protocols
Overview
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Product nameAnti-Glutamine Synthetase antibody
See all Glutamine Synthetase primary antibodies -
DescriptionRabbit polyclonal to Glutamine Synthetase
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IHC-Frmore details
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Species reactivityReacts with: Mouse, Rat, Monkey
Predicted to work with: Chicken, Hamster, Cow, Dog, Human, Pig, Cynomolgus monkey
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Immunogen
Synthetic peptide:
RTCLLNETGDEPFQYKN
, corresponding to C terminal amino acids 357-373 of Mouse Glutamine Synthetase -
Positive control
- Rat brain tissue, rat brain cytosolic fraction extract, cerebellum, kidney
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
PurityIon Exchange Chromatography
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Purification notesWhole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab49873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000. Predicted molecular weight: 42 kDa. | |
| IHC-P | 1/10000 - 1/20000. | |
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-Fr | 1/5000. |
Target
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FunctionThis enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
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Involvement in diseaseDefects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
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Sequence similaritiesBelongs to the glutamine synthetase family.
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Developmental stageExpressed during early fetal stages.
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Cellular localizationCytoplasm. Mitochondrion.
- Information by UniProt
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Database links
- Entrez Gene: 396489 Chicken
- Entrez Gene: 281199 Cow
- Entrez Gene: 2752 Human
- Entrez Gene: 14645 Mouse
- Entrez Gene: 396944 Pig
- Entrez Gene: 24957 Rat
- Omim: 138290 Human
- SwissProt: P16580 Chicken
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Alternative names
- cell proliferation-inducing protein 59 antibody
- GLNA antibody
- GLNA_HUMAN antibody
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Images
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Western blot - Anti-Glutamine Synthetase antibody (ab49873)All lanes :
Lane 2 : 10000
Lane 3 : 20000
Secondary
All lanes : Goat Anti-Rabbit IgG-Peroxidase
Predicted band size: 42 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Glutamine Synthetase antibody (ab49873)This image is courtesy of an Abreview submitted by Dr Zsuzsanna Kornyeiab49873 at 1/5000 staining cultured mouse astroglial cells by ICC/IF. The cells were paraformaldehyde fixed and permeabilized with TritonX100 before being blocked and stained with the antibody for 24 hours at 4°C. An Alexa-Fluor ® 594 conjugated chicken anti-rabbit antibody was used as the secondary.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody (ab49873)This image is courtesy of an Abreview submitted by Mr Carl HobbsGlutamine Synthetase antibody (ab49873) immunohistochemistry on rat brain and kidney tissue (formalin/PFA-fixed paraffin-embedded) sections. Rat cerebellum and rat kidney sections were incubated with ab49873 (1/21000) for 2h at RT. Antigen retrieval was performed by heat induction in citrate buffer pH6. The upper panel shows immunoreactivity in rat kidney, apart from convoluted tubule positivity (proximal: see asterisks) there is positivity within the glomerular capsule (perhaps the parietal epithelium: see arrows). In the lower image (cerebellum), apart from astrocytic glial cell body and process positivity, I note that what I think are oligodendrocytes in the grey matter, are also positive. In the corpus callosum of the same section (and all other major axonal tracks of this brain), linearly arranged cells are also positive: these are known to be oligodendrocytes.
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Protocols
References
This product has been referenced in:
- Zhou M et al. Non-cell-autonomous activation of IL-6/STAT3 signaling mediates FGF19-driven hepatocarcinogenesis. Nat Commun 8:15433 (2017). IHC-P ; Mouse . Read more (PubMed: 28508871) »
- Sidoryk-Wegrzynowicz M et al. Astrocytes in mouse models of tauopathies acquire early deficits and lose neurosupportive functions. Acta Neuropathol Commun 5:89 (2017). WB . Read more (PubMed: 29187256) »
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