Anti-Glutamine Synthetase antibody (ab49873)
Key features and details
- Rabbit polyclonal to Glutamine Synthetase
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Glutamine Synthetase antibody
See all Glutamine Synthetase primary antibodies -
Description
Rabbit polyclonal to Glutamine Synthetase -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Chicken, Hamster, Cow, Dog, Human, Pig, Cynomolgus monkey
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Immunogen
Synthetic peptide corresponding to Mouse Glutamine Synthetase aa 357-373 (C terminal).
Sequence:RTCLLNETGDEPFQYKN
Database link: P09606 -
Positive control
- WB: Rat brain whole extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Purification notes
Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab49873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000. Predicted molecular weight: 42 kDa.
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| Notes |
|---|
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WB
1/10000. Predicted molecular weight: 42 kDa. |
Target
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Function
This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts. -
Involvement in disease
Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid. -
Sequence similarities
Belongs to the glutamine synthetase family. -
Developmental stage
Expressed during early fetal stages. -
Cellular localization
Cytoplasm. Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 396489 Chicken
- Entrez Gene: 281199 Cow
- Entrez Gene: 2752 Human
- Entrez Gene: 396944 Pig
- Entrez Gene: 24957 Rat
- Omim: 138290 Human
- SwissProt: P16580 Chicken
- SwissProt: P15103 Cow
see all -
Alternative names
- cell proliferation-inducing protein 59 antibody
- Cgl2214 antibody
- GLNA antibody
see all
Images
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Western blot - Anti-Glutamine Synthetase antibody (ab49873)Lane 1 : Anti-Glutamine Synthetase antibody (ab49873) at 1/5000 dilution
Lane 2 : Anti-Glutamine Synthetase antibody (ab49873) at 1/10000 dilution
Lane 3 : Anti-Glutamine Synthetase antibody (ab49873) at 1/20000 dilution
All lanes : Rat brain whole extract
Secondary
All lanes : Goat Anti-Rabbit IgG-Peroxidase
Predicted band size: 42 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (51)
ab49873 has been referenced in 51 publications.
- Zhuang X et al. SHP-1 suppresses endotoxin-induced uveitis by inhibiting the TAK1/JNK pathway. J Cell Mol Med 25:147-160 (2021). PubMed: 33207073
- Fierros-Campuzano J et al. Irreversible hippocampal changes induced by high fructose diet in rats. Nutr Neurosci N/A:1-13 (2020). PubMed: 33269659
- Umezu T et al. Comprehensive analysis of liver and blood miRNA in precancerous conditions. Sci Rep 10:21766 (2020). PubMed: 33303811
- García-Bonilla M et al. Neocortical tissue recovery in severe congenital obstructive hydrocephalus after intraventricular administration of bone marrow-derived mesenchymal stem cells. Stem Cell Res Ther 11:121 (2020). PubMed: 32183876
- Ma R et al. Metabolic and non-metabolic liver zonation is established non-synchronously and requires sinusoidal Wnts. Elife 9:N/A (2020). PubMed: 32154783