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    glutamine-synthetase-antibody-ab49873.pdf

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Neuroscience Neurotransmitter Amino Acids Glutamate
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Anti-Glutamine Synthetase antibody (ab49873)

  • Datasheet
  • SDS
Reviews (12)Q&A (8)References (38)

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Western blot - Anti-Glutamine Synthetase antibody (ab49873)

    Key features and details

    • Rabbit polyclonal to Glutamine Synthetase
    • Suitable for: WB
    • Reacts with: Rat
    • Isotype: IgG

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    Overview

    • Product name

      Anti-Glutamine Synthetase antibody
      See all Glutamine Synthetase primary antibodies
    • Description

      Rabbit polyclonal to Glutamine Synthetase
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Rat
      Predicted to work with: Chicken, Hamster, Cow, Dog, Human, Pig, Cynomolgus monkey
    • Immunogen

      Synthetic peptide corresponding to Mouse Glutamine Synthetase aa 357-373 (C terminal).
      Sequence:

      RTCLLNETGDEPFQYKN


      Database link: P09606
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • WB: Rat brain whole extract.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.097% Sodium azide
      Constituent: 0.0268% PBS
    • Concentration information loading...
    • Purity

      Ion Exchange Chromatography
    • Purification notes

      Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurotransmitter
      • Amino Acids
      • Glutamate
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human Glutamine Synthetase protein (ab222354)

    Applications

    Our Abpromise guarantee covers the use of ab49873 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/10000. Predicted molecular weight: 42 kDa.

    Target

    • Function

      This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
    • Involvement in disease

      Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
    • Sequence similarities

      Belongs to the glutamine synthetase family.
    • Developmental stage

      Expressed during early fetal stages.
    • Cellular localization

      Cytoplasm. Mitochondrion.
    • Target information above from: UniProt accession P15104 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 396489 Chicken
      • Entrez Gene: 281199 Cow
      • Entrez Gene: 2752 Human
      • Entrez Gene: 396944 Pig
      • Entrez Gene: 24957 Rat
      • Omim: 138290 Human
      • SwissProt: P16580 Chicken
      • SwissProt: P15103 Cow
      • SwissProt: Q8HZM5 Dog
      • SwissProt: P15104 Human
      • SwissProt: P46410 Pig
      • SwissProt: P09606 Rat
      • Unigene: 518525 Human
      • Unigene: 2204 Rat
      see all
    • Alternative names

      • cell proliferation-inducing protein 59 antibody
      • Cgl2214 antibody
      • GLNA antibody
      • GLNA_HUMAN antibody
      • GLNS antibody
      • GLUL antibody
      • Glutamate ammonia ligase antibody
      • Glutamate decarboxylase antibody
      • Glutamate--ammonia ligase antibody
      • glutamine synthase antibody
      • Glutamine synthetase antibody
      • glutamine synthetase I antibody
      • GS antibody
      • PIG 43 antibody
      • PIG 59 antibody
      • PIG43 antibody
      • PIG59 antibody
      • Proliferation inducing protein 43 antibody
      see all

    Images

    • Western blot - Anti-Glutamine Synthetase antibody (ab49873)
      Western blot - Anti-Glutamine Synthetase antibody (ab49873)
      Lane 1 : Anti-Glutamine Synthetase antibody (ab49873) at 1/5000 dilution
      Lane 2 : Anti-Glutamine Synthetase antibody (ab49873) at 1/10000 dilution
      Lane 3 : Anti-Glutamine Synthetase antibody (ab49873) at 1/20000 dilution

      All lanes : Rat brain whole extract

      Secondary
      All lanes : Goat Anti-Rabbit IgG-Peroxidase

      Predicted band size: 42 kDa

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (38)

    Publishing research using ab49873? Please let us know so that we can cite the reference in this datasheet.

    ab49873 has been referenced in 38 publications.

    • García-Bonilla M  et al. Neocortical tissue recovery in severe congenital obstructive hydrocephalus after intraventricular administration of bone marrow-derived mesenchymal stem cells. Stem Cell Res Ther 11:121 (2020). PubMed: 32183876
    • Ma R  et al. Metabolic and non-metabolic liver zonation is established non-synchronously and requires sinusoidal Wnts. Elife 9:N/A (2020). PubMed: 32154783
    • Scheidecker B  et al. Induction of in vitro Metabolic Zonation in Primary Hepatocytes Requires Both Near-Physiological Oxygen Concentration and Flux. Front Bioeng Biotechnol 8:524 (2020). PubMed: 32656187
    • Chen Y  et al. Fine-scale visualizing the hierarchical structure of mouse biliary tree with fluorescence microscopy method. Biosci Rep 40:N/A (2020). PubMed: 32364232
    • Pereiro X  et al. Optimization of a Method to Isolate and Culture Adult Porcine, Rats and Mice Müller Glia in Order to Study Retinal Diseases. Front Cell Neurosci 14:7 (2020). PubMed: 32082123
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 20 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Glutamine Synthetase antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Adult mouse dissociated dorsal root ganglia prepar)
    Permeabilization
    Yes - 0.3% Triton X100 in PBS (0.1M, pH7.4) 1h, RT (blocking step)
    Specification
    Adult mouse dissociated dorsal root ganglia prepar
    Blocking step
    Donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Stephen Woodhams

    Verified customer

    Submitted Oct 13 2020

    Flow Cytometry abreview for Anti-Glutamine Synthetase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (Hepatocytes)
    Permeabilization
    Yes - Saponin
    Gating Strategy
    1. Single cells (FSC-H vs. FSC-A) 2. Viable cells (SSC-A vs. FSC-A) 3. GS positive cells
    Specification
    Hepatocytes
    Preparation
    Cell harvesting/tissue preparation method: Collagenase perfusion
    Sample buffer: Perfusion buffer
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 20 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glutamine Synthetase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: CC1 Ventana (high pH)
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    BSA as blocking agent for 40 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Enrico Radaelli

    Verified customer

    Submitted Aug 21 2017

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Glutamine Synthetase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Jugular nodose ganglion complex (JNC))
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Jugular nodose ganglion complex (JNC)
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 5 · Temperature: RT°C
    Fixative
    Zamboni
    Read More

    Mr. Duc Dung Le

    Verified customer

    Submitted Apr 20 2015

    IHC - Wholemount abreview for Anti-Glutamine Synthetase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Retina)
    Specification
    Retina
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 04 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glutamine Synthetase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver)
    Specification
    Liver
    Fixative
    Formalin
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 12 2013

    Question

    Antibody has been stored in aliquots at -80C for a year. It has been working great but the most recent aliquots are not working at all.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 18 2012

    Answer

    Thank you for your call today and for letting us know about this issue.
    I've created a discount code that can be used to receive 20% off a vial of ab49873. Please include the code below with your order to redeem this offer:
    Discount Code: ***
    Expires: August 18, 2012
    I hope that this will be helpful, but please let me know if you have any questions or if there is anything else that we can do for you. Have a nice day.

    Read More

    Abcam Scientific Support

    Answered on Jul 18 2012

    Question

    phone call: use these antibody in IHC-P and human

    Read More

    Abcam community

    Verified customer

    Asked on Apr 03 2012

    Answer

    Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

    Wie am Telefon diskutiert, ist unseres Wissens nach wurde der ab49873 bisher noch nicht inauf humanem Gewebegetestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von ab49873 abdecken, und gegen eine erneute Bestellung eines weiterenprimären Antikörpersvon uns verrechnet werden.

    Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

    1.) Bestätigen Sie mir bitte, dass Sie ab49873 auf humanem Gewebetesten möchten.

    2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

    3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

    4.) Testen Sie den Antikörper auf humanem Gewebe.

    5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
    Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
    https://www.abcam.com/abreviews

    6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können einen anderenprimären Antikörperbei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode und die Bestellnummer von ab49873 bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

    Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

    Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
    https://www.abcam.com/collaborationdiscount

    Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

    Read More

    Abcam Scientific Support

    Answered on Apr 03 2012

    Question

    Dear,

    Please ignore the last e mail as I did not realise that 2 antibodies were included in one shipment ab60709 and ab 91073,

    Thank you very much again Karin,

    Kind Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Mar 29 2012

    Answer

    That's no problem. I am sorry for the confusion, I should have made it clear to you that they would be arriving in the same box.

    If I can help in any other way please do let me know. Otherwise, I wish you all the best with your research.

    Read More

    Abcam Scientific Support

    Answered on Mar 29 2012

    Question

    Thank you very much,

    Read More

    Abcam community

    Verified customer

    Asked on Mar 27 2012

    Answer

    That is no problem. If you have any problems in the future, please do not hesitate to contact us again.

    Until then, I wish you all the best with your research.

    Read More

    Abcam Scientific Support

    Answered on Mar 27 2012

    1-10 of 20 Abreviews or Q&A

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