Product nameAnti-Glutamine Synthetase antibody
See all Glutamine Synthetase primary antibodies
DescriptionRabbit polyclonal to Glutamine Synthetase
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat, Monkey
Predicted to work with: Chicken, Hamster, Cow, Dog, Human, Pig, Cynomolgus monkey
- Rat brain tissue, rat brain cytosolic fraction extract, cerebellum, kidney
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
PurityIon Exchange Chromatography
Purification notesWhole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
Our Abpromise guarantee covers the use of ab49873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000. Predicted molecular weight: 42 kDa.|
|IHC-P||1/10000 - 1/20000.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionThis enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
Involvement in diseaseDefects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
Sequence similaritiesBelongs to the glutamine synthetase family.
Developmental stageExpressed during early fetal stages.
Cellular localizationCytoplasm. Mitochondrion.
- Information by UniProt
- cell proliferation-inducing protein 59 antibody
- GLNA antibody
- GLNA_HUMAN antibody
All lanes :
Lane 2 : 10000
Lane 3 : 20000
All lanes : Goat Anti-Rabbit IgG-Peroxidase
Predicted band size: 42 kDa
ab49873 at 1/5000 staining cultured mouse astroglial cells by ICC/IF. The cells were paraformaldehyde fixed and permeabilized with TritonX100 before being blocked and stained with the antibody for 24 hours at 4°C. An Alexa-Fluor ® 594 conjugated chicken anti-rabbit antibody was used as the secondary.
Glutamine Synthetase antibody (ab49873) immunohistochemistry on rat brain and kidney tissue (formalin/PFA-fixed paraffin-embedded) sections. Rat cerebellum and rat kidney sections were incubated with ab49873 (1/21000) for 2h at RT. Antigen retrieval was performed by heat induction in citrate buffer pH6. The upper panel shows immunoreactivity in rat kidney, apart from convoluted tubule positivity (proximal: see asterisks) there is positivity within the glomerular capsule (perhaps the parietal epithelium: see arrows). In the lower image (cerebellum), apart from astrocytic glial cell body and process positivity, I note that what I think are oligodendrocytes in the grey matter, are also positive. In the corpus callosum of the same section (and all other major axonal tracks of this brain), linearly arranged cells are also positive: these are known to be oligodendrocytes.
This product has been referenced in:
- Zhou M et al. Non-cell-autonomous activation of IL-6/STAT3 signaling mediates FGF19-driven hepatocarcinogenesis. Nat Commun 8:15433 (2017). IHC-P ; Mouse . Read more (PubMed: 28508871) »
- Sidoryk-Wegrzynowicz M et al. Astrocytes in mouse models of tauopathies acquire early deficits and lose neurosupportive functions. Acta Neuropathol Commun 5:89 (2017). WB . Read more (PubMed: 29187256) »