The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000. Predicted molecular weight: 42 kDa.
1/10000 - 1/20000.
Use at an assay dependent concentration.
This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
Involvement in disease
Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
Western blot - Anti-Glutamine Synthetase antibody (ab49873)
All lanes :
Lane 2 : 10000 Lane 3 : 20000
Secondary All lanes : Goat Anti-Rabbit IgG-Peroxidase
Predicted band size: 42 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Glutamine Synthetase antibody (ab49873)This image is courtesy of an Abreview submitted by Dr Zsuzsanna Kornyei
ab49873 at 1/5000 staining cultured mouse astroglial cells by ICC/IF. The cells were paraformaldehyde fixed and permeabilized with TritonX100 before being blocked and stained with the antibody for 24 hours at 4°C. An Alexa-Fluor ® 594 conjugated chicken anti-rabbit antibody was used as the secondary.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody (ab49873)This image is courtesy of an Abreview submitted by Mr Carl Hobbs
Glutamine Synthetase antibody (ab49873) immunohistochemistry on rat brain and kidney tissue (formalin/PFA-fixed paraffin-embedded) sections. Rat cerebellum and rat kidney sections were incubated with ab49873 (1/21000) for 2h at RT. Antigen retrieval was performed by heat induction in citrate buffer pH6. The upper panel shows immunoreactivity in rat kidney, apart from convoluted tubule positivity (proximal: see asterisks) there is positivity within the glomerular capsule (perhaps the parietal epithelium: see arrows). In the lower image (cerebellum), apart from astrocytic glial cell body and process positivity, I note that what I think are oligodendrocytes in the grey matter, are also positive. In the corpus callosum of the same section (and all other major axonal tracks of this brain), linearly arranged cells are also positive: these are known to be oligodendrocytes. .
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Sidoryk-Wegrzynowicz M et al. Astrocytes in mouse models of tauopathies acquire early deficits and lose neurosupportive functions. Acta Neuropathol Commun5:89 (2017).
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