Immunohistochemistry (Frozen sections) abreview for Anti-Glutamine Synthetase antibody

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (early postnatal brain section)
Specification
early postnatal brain section
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Other product details

Dilution
1/5000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS+1% serum

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit IgG Highly cross-absorbed
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Dilution
1/500

Additional data

Additional Notes
The most common astrocyte marker GFAP does not stain a lot of astrocytes in the cortex, especially in young postnatal animals. Glutamine Synthetase solved this problem and the staining is very good with little background.

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Submitted Jul 16 2008

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