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Host species: Goat
The dilution factor of 1/21,000 for ab49873 is astounding: based upon Dr Broadstock's advice that this Ab lot can be diluted out to 1/10K, I made a 1/70 stock of the sample and made a range of manageable dilutions of this (making a 1/10K dilution of a neat stock invites greater dilution errors). Note that in the kidney section, apart from convoluted tubule positivity (proximal: see asterisks) there is positivity within the glomerular capsule (perhaps the parietal epithelium: see arrows).
In the lower image (cerebellum), apart from astrocytic glial cell body and process positivity, I note that what I think are oligodendrocytes in the grey matter, are also positive. In the corpus callosum of the same section (and all other major axonal tracks of this brain), linearly arranged cells are also positive: these are known to be oligodendrocytes.
More images, as usual, should shortly be uploaded to http://www.immunoportal.com/
Mr. Carl Hobbs
Submitted Apr 01 2009