Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glutamine Synthetase antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney and cerebellum)
Specification
Kidney and cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid 10mM pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C

Other product details

Dilution
1/21000
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide

Secondary antibody

Name
Non-Abcam antibody was used: anti rabbit Igs
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200

Additional data

Additional Notes
I thank my colleague Dr Martin Broadstock for a sample and also his advice regarding Glutamine Synthetase distribution in brain. I rate ab49873 as excellent because it gives me very precise immunolabelling (signal:noise ratio).

The dilution factor of 1/21,000 for ab49873 is astounding: based upon Dr Broadstock's advice that this Ab lot can be diluted out to 1/10K, I made a 1/70 stock of the sample and made a range of manageable dilutions of this (making a 1/10K dilution of a neat stock invites greater dilution errors). Note that in the kidney section, apart from convoluted tubule positivity (proximal: see asterisks) there is positivity within the glomerular capsule (perhaps the parietal epithelium: see arrows).

In the lower image (cerebellum), apart from astrocytic glial cell body and process positivity, I note that what I think are oligodendrocytes in the grey matter, are also positive. In the corpus callosum of the same section (and all other major axonal tracks of this brain), linearly arranged cells are also positive: these are known to be oligodendrocytes.

More images, as usual, should shortly be uploaded to http://www.immunoportal.com/

Mr. Carl Hobbs

Verified customer

Submitted Apr 01 2009

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