Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glutamine Synthetase antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Cerebellum)
Specification
Cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C

Other product details

Dilution
1/5250
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide

Secondary antibody

Name
Non-Abcam antibody was used: anti rabbit IgGs
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200

Additional data

Additional Notes
My apologies for the poor quality of the histology of the image: the fixation was inadequate causing increased loss of structure during HIER
However, positivity seems to be identical to that in rat/mouse cerebellum.
NB: I keep a 4C 1/70 stock of this Ab reagent: I diluted it 1/75 to get the results seen in the image.
Abcam response
Many thanks for your feedback on ab49873 (Glutamine Synthetase antibody) in monkey cerebellum in IHC-P; this is the first report we have received of this antibody's performance with monkey tissue.

Mr. Carl Hobbs

Verified customer

Submitted Apr 21 2010

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