Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glutamine Synthetase antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain cortex and colon)
Specification
Brain cortex and colon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C

Other product details

Dilution
1/4000
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide

Secondary antibody

Name
Non-Abcam antibody was used: anti rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200

Additional data

Additional Notes
Upper image shows positivity in brain cortex: black arrowheads indicates striking positivity in two Astrocytes...or are they Microglia?
It is difficult for me to confirm in tissues sampled Post-mortem due to autolytic changes but, they appear to be Astrocytes.
Lower image of colon shows a striking positivity in cells within the Lamina propria of the mucosa.....Macrophages?
I do not know if this enzyme is in macrophages but, from a brief literature search, it is reported to be present in liver Kuppfer cells ( specialised macrophages).
Dilution factors will vary: each Lab must titrate the Ab to find the optimal factor most suitable to their application.

NB: there is a small but obvious granular positivity in the apical cytoplasm of the mucosal cells.

Mr. Carl Hobbs

Verified customer

Submitted Jan 22 2010

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