Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glutamine Synthetase antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 23°C

Other product details

Dilution
1/10000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: 1% BSA in TBS

Secondary antibody

Name
Non-Abcam antibody was used: Jackson Immunoresearch Donkey anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/500

Additional data

Additional Notes
The Vectastain RTU Label from Vector Labs was used for 30 minutes after the secondary.

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Submitted Mar 12 2013

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