Immunocytochemistry/ Immunofluorescence abreview for Anti-Glutamine Synthetase antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cultured Cells (astroglia)
Specification
astroglia
Fixative
Paraformaldehyde
Permeabilization
Yes - TritonX100 0.1%
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Dilution
1/5000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: +4°C · Diluent: PBS + 3%FCS

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit Alexa594
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/800

Additional data

Additional Notes
This antibody works even in 1/10000, if developed with anti-rabbit Alexa488. It is also great for western blot !

Dr. Zsuzsanna Kornyei

Verified customer

Submitted Oct 29 2007

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