Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (ab64613)
Key features and details
- Mouse monoclonal [3B6] to Glutamine Synthetase - BSA and Azide free
- Suitable for: WB, ICC/IF, Flow Cyt, Sandwich ELISA, IHC-P
- Reacts with: Mouse, Rat, Human, Drosophila melanogaster, Zebrafish
- Isotype: IgG1
Overview
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Product name
Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free
See all Glutamine Synthetase primary antibodies -
Description
Mouse monoclonal [3B6] to Glutamine Synthetase - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt, Sandwich ELISA, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Drosophila melanogaster, Zebrafish
Predicted to work with: Horse, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Cynomolgus monkey, Opossum, Chinese hamster -
Immunogen
Recombinant fragment corresponding to Human Glutamine Synthetase aa 274-373.
Sequence:IEKLSKRHQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASI RIPRTVGQEKKGYFEDRRPSANCDPFSVTEALIRTCLLNETGDEPFQYKN
Database link: P15104 -
Positive control
- IHC-P: Human tonsil tissue. WB: Jurkat whole cell lysate; Raw 264.7 cells, HepG2 cell, PC-12, Zebrafish brain, liver, skeletal muscle homogenate ICC: PC12 cells. Flow Cyt: Jurkat cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 22/03/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3B6 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 42 kDa).
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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Sandwich ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 42 kDa). |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Sandwich ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts. -
Involvement in disease
Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid. -
Sequence similarities
Belongs to the glutamine synthetase family. -
Developmental stage
Expressed during early fetal stages. -
Cellular localization
Cytoplasm. Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 396489 Chicken
- Entrez Gene: 281199 Cow
- Entrez Gene: 2752 Human
- Entrez Gene: 14645 Mouse
- Entrez Gene: 396944 Pig
- Entrez Gene: 24957 Rat
- Entrez Gene: 398556 Xenopus laevis
- Omim: 138290 Human
see all -
Alternative names
- cell proliferation-inducing protein 59 antibody
- Cgl2214 antibody
- GLNA antibody
see all
Images
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IHC image of ab64613 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64613, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.This image was generated using the ascites version of the product.
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Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (ab64613) at 1 µg/ml + Jurkat cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 42 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?This image was generated using the ascites version of the product.
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ICC image of ab64613 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64613, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
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Overlay histogram showing Jurkat cells stained with ab64613 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab64613, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
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All lanes : Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (ab64613)
Lane 1 : Glutamine Synthetase transfected lysate
Lane 2 : Non-transfected lysate
Predicted band size: 42 kDaThis image was generated using the ascites version of the product.
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Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (ab64613) + Raw 264.7 cell line
Predicted band size: 42 kDaThis image was generated using the ascites version of the product.
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Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (ab64613) + HepG2 cell lysate
Predicted band size: 42 kDaThis image was generated using the ascites version of the product.
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Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (ab64613) + PC-12 cell lysate
Predicted band size: 42 kDaThis image was generated using the ascites version of the product.
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All lanes : Anti-Glutamine Synthetase antibody [3B6] - BSA and Azide free (ab64613) at 0.5 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Zebrafish liver homogenate at 20 µg
Lane 4 : Zebrafish skeletal muscle homogenate at 20 µg
Lane 5 : JURKAT (Human T cell lymphoblast-like cell line) whole cell lysate at 20 µg
Secondary
All lanes : Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 4 minutesThis image was generated using the ascites version of the product.
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Detection limit for recombinant GST tagged Glutamine Synthetase is approximately 0.03ng/ml as a capture antibody.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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Datasheet download
References (48)
ab64613 has been referenced in 48 publications.
- Wei W et al. SIRT4 Is Highly Expressed in Retinal Müller Glial Cells. Front Neurosci 16:840443 (2022). PubMed: 35185463
- Wang Y et al. Interleukin 33-mediated inhibition of A-type K+ channels induces sensory neuronal hyperexcitability and nociceptive behaviors in mice. Theranostics 12:2232-2247 (2022). PubMed: 35265208
- Tang G et al. Naringin Relieves Diabetic Cardiac Autonomic Neuropathy Mediated by P2Y14 Receptor in Superior Cervical Ganglion. Front Pharmacol 13:873090 (2022). PubMed: 35529431
- Tang Y et al. Nicotinamide ameliorates energy deficiency and improves retinal function in Cav-1-/- mice. J Neurochem 157:550-560 (2021). PubMed: 33305362
- Van Houcke J et al. Aging impairs the essential contributions of non-glial progenitors to neurorepair in the dorsal telencephalon of the Killifish Nothobranchius furzeri. Aging Cell 20:e13464 (2021). PubMed: 34428340