Western blot abreview for Anti-Glutamine Synthetase antibody

Excellent
Abreviews
Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (Drosophila S2 cell)
Loading amount
20 µg
Specification
Drosophila S2 cell
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/3000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C

Secondary antibody

Name
Non-Abcam antibody was used: alpha-mouse IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000

Detection

Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 43 kDa

Additional data

Additional Notes
lysis buffer : RIPA lysis buffer

Drosophila S2 cell whole cell lysate

Size marker : SeeBlue plus 2 (Invitrogen)

Membrane : ECL hybond+

Mr. Myoung Sup Shim

Verified customer

Submitted Apr 14 2009

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