Immunohistochemistry (Frozen sections) abreview for Anti-Glutamine Synthetase antibody

Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

Other product details

Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking solution

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 488 - conjugated goat anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200

Additional data

Additional Notes
The antibody was applied on 16 µm thick sections of adult brown ghost knifefish brain. The image shows labeled radial glia in the optic tectum.
The labeling appears specific, however relatively high levels of background can be seen, especially at lower magnifications.

Dr. Ruxandra Sirbulescu

Verified customer

Submitted Nov 28 2011

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