Western blot abreview for Anti-Glutamine Synthetase antibody

Average
Abreviews
Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Dilution
1/700
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 2.5% milk in PBS-T

Secondary antibody

Name
Non-Abcam antibody was used: Alexa488-conjugated goat anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Detection

Detection method
fluorescence scanning
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 42 kDa Non-specific: 30, 18 kDa

Additional data

Additional Notes
The antibody identifies a band at the predicted molecular weight, however numerous other bands are also detected, potentially degradation products.

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Submitted Jul 03 2012

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