Immunohistochemistry (Frozen sections)

Poor
Abreviews
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Zebrafish Tissue sections (Retina, Muller glia cells)
Specification
Retina, Muller glia cells
Permeabilization
Yes - 0.1% TritonX
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Block solution
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Alexa 647 conjugated goat anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Dilution
1/1000

Additional data

Additional Notes
No labelling in the retina at 4 days post fertilization
Abcam response
Thank you for submitting an Abreview. ab64613 does not seem to work under these experimental conditions. Thank you for your time, this information will surely be helpful for customers.

Dr. Ryan Macdonald

Verified customer

Submitted May 28 2013

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