Immunocytochemistry/ Immunofluorescence abreview for Anti-Glutamine Synthetase antibody

Inconclusive
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Bos taurus Cell (Dorsal root ganglia (spinal) cell culture, and ast)
Permeabilization
Yes - triton x 0.5%
Specification
Dorsal root ganglia (spinal) cell culture, and ast
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Methanol

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST+10%NGS
Concentration
5 µg/ml

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: goat anti mouse IgG Conjugation: Alexa Fluor­ 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 488

Additional data

Additional Notes
I would like to characterize the non neuronal elements of my culture. To differentiate satellite from other cells I need a monoclonal antibody. Unfortunately the ab 64613 to gluthamine synthetase did not work. I have tried different concentration from 1 ug/m to 10 ug/ml. I will send some pictures in the next days. Thank you for your support. We also tried to characterize bovine astrocyte cells from primary cultures, and we used the ab64613 together with GFAP in order to identify the astrocytic cells. We could not get a signal with this antibody, although we tried different fixation methods (methanol, aceton/methanol, PFA).
Abcam response
Thank you for participating in our AbTrial testing program for untested species or applications. Based on this feedback it appears that ab64613 may not be suitable for Bos taurus reactivity.

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Submitted Feb 01 2017

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