Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Glutamine Synthetase antibody

Excellent
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain - Hippocampus)
Specification
Brain - Hippocampus
Fixative
Paraformaldehyde

Other product details

Dilution
1/3000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBST

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti rabbit Alexa fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

Additional Notes
This antibody produced a beautiful staining in astrocytic-like cells. the picture shows the staining obtained at the level of the hippocampus, using the objective X20 (left picture) and at higher power magnification, using the objective X40 (right picture).

The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

Dr. Sophie Pezet

Verified customer

Submitted Sep 08 2010

Sign up