Non-Abcam antibody was used: Goat anti rabbit Alexa fluor 488 Host species: Goat Clonality: Polyclonal Conjugation: Alexa Fluor® 488
This antibody produced a beautiful staining in astrocytic-like cells. the picture shows the staining obtained at the level of the hippocampus, using the objective X20 (left picture) and at higher power magnification, using the objective X40 (right picture).
The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.
Dr. Sophie Pezet
Submitted Sep 08 2010
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