The antibody was applied on 16 µm thick sections of brown ghost knifefish spinal cord and brain. 2% paraformaldehyde was used during perfusion and for post-fixation of the tissue before cryosectioning. No specific staining could be detected in either tissue sample.
Thank you very much for testing this antibody in Apteronotus. We are sorry that it apparently does not work with this species under the given conditions.
Dr. Ruxandra Sirbulescu
Submitted Nov 14 2011
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