Immunohistochemistry (Frozen sections) abreview for Anti-Glutamine Synthetase antibody

Poor
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Brain, spinal cord)
Specification
Brain, spinal cord
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

Other product details

Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking solution

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 488 - conjugated goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200

Additional data

Additional Notes
The antibody was applied on 16 µm thick sections of brown ghost knifefish spinal cord and brain. 2% paraformaldehyde was used during perfusion and for post-fixation of the tissue before cryosectioning. No specific staining could be detected in either tissue sample.
Abcam response
Thank you very much for testing this antibody in Apteronotus. We are sorry that it apparently does not work with this species under the given conditions.

Dr. Ruxandra Sirbulescu

Verified customer

Submitted Nov 14 2011

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