Immunohistochemistry (Frozen sections)

Below Average
Abreviews
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Zebrafish Tissue sections (Retina, Muller Cell)
Specification
Retina, Muller Cell
Permeabilization
No
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Block solution
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Alexa 488 conjugated goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

Additional Notes
Weak staining in Muller cells, non-specific stain in the inner nuclear layer and ganglion cell layer in 4dpf zebrafish embryo retina sections.
Abcam response
Thank you for submitting an Abreview. ab73593 had not been tested in zebrafish previously and apparently does not seem to work well in this species, under these experimental conditions. Thank you for your time, this information will surely be helpful for customers.

Dr. Ryan Macdonald

Verified customer

Submitted May 28 2013

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