Key features and details
- Rabbit polyclonal to Glutamine Synthetase
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-Glutamine Synthetase antibody
See all Glutamine Synthetase primary antibodies
DescriptionRabbit polyclonal to Glutamine Synthetase
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Chicken, Cow, Dog, Human, Pig, Zebrafish, Cynomolgus monkey, Chinese hamster
Synthetic peptide corresponding to Mouse Glutamine Synthetase aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab93439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).|
FunctionThis enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
Involvement in diseaseDefects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
Sequence similaritiesBelongs to the glutamine synthetase family.
Developmental stageExpressed during early fetal stages.
Cellular localizationCytoplasm. Mitochondrion.
- Information by UniProt
- cell proliferation-inducing protein 59 antibody
- Cgl2214 antibody
- GLNA antibody
All lanes : Anti-Glutamine Synthetase antibody (ab93439) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Cerebellum (Mouse) Tissue Lysate
Lane 4 : Cerebellum (Rat) Tissue Lysate
Lane 5 : Kidney (Mouse) Tissue Lysate
Lane 6 : Kidney (Rat) Tissue Lysate
Lane 7 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
ab93439 has been referenced in 4 publications.
- Mitra S et al. Dual regulation of lin28a by Myc is necessary during zebrafish retina regeneration. J Cell Biol 218:489-507 (2019). PubMed: 30606747
- Mitra S et al. Histone Deacetylase-Mediated Müller Glia Reprogramming through Her4.1-Lin28a Axis Is Essential for Retina Regeneration in Zebrafish. iScience 7:68-84 (2018). PubMed: 30267687
- Morales I et al. Striatal astrocytes engulf dopaminergic debris in Parkinson's disease: A study in an animal model. PLoS One 12:e0185989 (2017). PubMed: 29028815
- Morales I et al. The astrocytic response to the dopaminergic denervation of the striatum. J Neurochem 139:81-95 (2016). PubMed: 27230040