Product nameAnti-Glutamine Synthetase antibody
See all Glutamine Synthetase primary antibodies
DescriptionRabbit polyclonal to Glutamine Synthetase
Tested applicationsSuitable for: WB, IHC-Fr, IHC-FoFrmore details
Species reactivityReacts with: Mouse, Rat, Zebrafish
Predicted to work with: Chicken, Cow, Dog, Human, Pig, Cynomolgus monkey, Chinese hamster
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse Glutamine Synthetase.
- This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Rat Brain; Mouse Cerebellum; Rat Cerebellum; Mouse Kidney; Rat Kidney; Mouse Liver.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab93439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).|
FunctionThis enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
Involvement in diseaseDefects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
Sequence similaritiesBelongs to the glutamine synthetase family.
Developmental stageExpressed during early fetal stages.
Cellular localizationCytoplasm. Mitochondrion.
- Information by UniProt
- cell proliferation-inducing protein 59 antibody
- GLNA antibody
- GLNA_HUMAN antibody
ab93439 staining Glutamine Synthetase in zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5% BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab93439 at 1/100 in blocking solution for 16h at 4°C. An alexa 647 conjugated goat anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. Glutamine Synthetase expression can be observed in Muller cells (in purple).
All lanes : Anti-Glutamine Synthetase antibody (ab93439) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Cerebellum (Mouse) Tissue Lysate
Lane 4 : Cerebellum (Rat) Tissue Lysate
Lane 5 : Kidney (Mouse) Tissue Lysate
Lane 6 : Kidney (Rat) Tissue Lysate
Lane 7 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
IHC-FoFr image of Glutamine Synthetase staining on rat brain sections using ab93439 (1:1000). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.
This product has been referenced in:
- Mitra S et al. Dual regulation of lin28a by Myc is necessary during zebrafish retina regeneration. J Cell Biol 218:489-507 (2019). Read more (PubMed: 30606747) »
- Mitra S et al. Histone Deacetylase-Mediated Müller Glia Reprogramming through Her4.1-Lin28a Axis Is Essential for Retina Regeneration in Zebrafish. iScience 7:68-84 (2018). Read more (PubMed: 30267687) »