Anti-Glutamine Synthetase antibody (Biotin) (ab34545)
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Overview
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Product nameAnti-Glutamine Synthetase antibody (Biotin)
See all Glutamine Synthetase primary antibodies -
DescriptionGoat polyclonal to Glutamine Synthetase (Biotin)
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Host speciesGoat
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ConjugationBiotin
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Tested applicationsSuitable for: WB, ELISA, ICC/IF, Dot blotmore details
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Species reactivityReacts with Microbia.
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Immunogen
Full length protein (Microbial)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium Azide
Constituents: 0.15% Sodium chloride, 0.02% Potassium phosphate, 10mg/ml BSA. pH 7.2 -
Concentration information loading... -
PurityIgG fraction
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Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab34545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| ELISA | ||
| ICC/IF | ||
| Dot blot |
Application notes
Dot: Use at an assay dependent dilution.
ELISA: 1/4000 - 1/20000. This product has been assayed against 1.0?g of Glutamine Synthetase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature.
ICC/IF: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 42 kDa.
Suitable for antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/4000 - 1/20000. This product has been assayed against 1.0?g of Glutamine Synthetase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature.
ICC/IF: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 42 kDa.
Suitable for antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionThis enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
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Involvement in diseaseDefects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
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Sequence similaritiesBelongs to the glutamine synthetase family.
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Developmental stageExpressed during early fetal stages.
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Cellular localizationCytoplasm. Mitochondrion.
- Information by UniProt
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Alternative names
- cell proliferation-inducing protein 59 antibody
- GLNA antibody
- GLNA_HUMAN antibody
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Datasheets and documents
References
ab34545 has not yet been referenced specifically in any publications.
