Product nameAnti-Glutamine Synthetase antibody [EPR13022(B)]
See all Glutamine Synthetase primary antibodies
DescriptionRabbit monoclonal [EPR13022(B)] to Glutamine Synthetase
Tested applicationsSuitable for: IHC-Fr, WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Glutamine Synthetase aa 250-350 (Cysteine residue). The exact sequence is proprietary.
Database link: P15104
- WB: Human fetal liver lysate, mouse and rat spleen lysate Jurkat, HAP1 and HeLa whole cell lysate (ab150035); IHC-P: Human glioma and liver tissues, mouse liver tissue; IHC-Fr: Rat and Mouse cerebrum.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab176562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 42 kDa.|
|IHC-P||1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.
For unpurified use at 1/100-1/250.
FunctionThis enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
Involvement in diseaseDefects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
Sequence similaritiesBelongs to the glutamine synthetase family.
Developmental stageExpressed during early fetal stages.
Cellular localizationCytoplasm. Mitochondrion.
- Information by UniProt
- cell proliferation-inducing protein 59 antibody
- GLNA antibody
- GLNA_HUMAN antibody
All lanes : Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab176562) at 1 µg
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : GLUL knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 42 kDa
Lanes 1 - 2: Merged signal (red and green). Green - ab176562 observed at 42 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab176562 was shown to recognize Glutamine Synthetase in wild-type HAP1 cells as signal was lost at the expected MW in GLUL knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GLUL knockout samples were subjected to SDS-PAGE. Ab176562 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilised mouse cerebrum tissue staining glutamine synthetase with ab176562 at 1/250 dilution, followed by alexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). DAPI was used as a nuclear counterstain.
Positive staining on mouse cerebrum (PMID: 23895693).
Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human glioma tissue labeling Glutamine Synthetase with unpurified ab176562 at a 1/100 dilution.
All lanes : Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab176562) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal liver lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 42 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human glioma tissue sections labeling Glutamine Synthetase with purified ab176562 at 1:500 dilution (0.18 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using citrate Buffer, pH6.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilised rat cerebrum tissue staining glutamine synthetase with ab176562 at 1/250 dilution, followed by alexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). DAPI was used as a nuclear counterstain.
Positive staining on rat cerebrum (PMID: 23895693).
Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human liver tissue labeling Glutamine Synthetase with unpurified ab176562 at a 1/100 dilution.
All lanes : Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab176562) at 1/2000 dilution (purified)
Lane 1 : Human fetal liver lysates at 20 µg
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Lane 3 : Mouse spleen lysates at 20 µg
Lane 4 : Rat spleen lysates at 20 µg
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Blocking and diluting buffer: 2% BSA/TBST
Western blot analysis on Immunoprecipitation pellet from either 1) Human fetal liver lysate, or 2) 1xPBS (negative control); showing Glutamine Synthetase, with unpurified ab176562, diluted in 1% BSA, at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
ab176562 staining Glutamine Synthetasein Mouse Liver tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, blocked with PB ab64226 for 10 minutes at room temperature and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/200) for 30 minutes. A HRP-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.
This product has been referenced in:
- Femenía T et al. Disrupted Neuroglial Metabolic Coupling after Peripheral Surgery. J Neurosci 38:452-464 (2018). Read more (PubMed: 29175959) »
- Wang L et al. Increased glutamine anabolism sensitizes non-small cell lung cancer to gefitinib treatment. Cell Death Discov 5:24 (2018). Read more (PubMed: 30109143) »