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Synthetic peptide within Human Glutamine Synthetase aa 250-350 (Cysteine residue). The exact sequence is proprietary.
Database link: P15104
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab176562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 42 kDa.|
|IHC-P||1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.
For unpurified use at 1/100-1/250.
Lanes 1 - 2: Merged signal (red and green). Green - ab176562 observed at 42 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab176562 was shown to recognize Glutamine Synthetase in wild-type HAP1 cells as signal was lost at the expected MW in GLUL knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GLUL knockout samples were subjected to SDS-PAGE. Ab176562 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human glioma tissue labeling Glutamine Synthetase with unpurified ab176562 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human glioma tissue sections labeling Glutamine Synthetase with purified ab176562 at 1:500 dilution (0.18 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using citrate Buffer, pH6.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human liver tissue labeling Glutamine Synthetase with unpurified ab176562 at a 1/100 dilution.
Blocking and diluting buffer: 2% BSA/TBST
Flow cytometry analysis of permeabilized HeLa cells labeling Glutamine Synthetase (red) with unpurified ab176562 at a 1/10 dilution, or negative control rabbit IgG (green).
Western blot analysis on Immunoprecipitation pellet from either 1) Human fetal liver lysate, or 2) 1xPBS (negative control); showing Glutamine Synthetase, with unpurified ab176562, diluted in 1% BSA, at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
ab176562 staining Glutamine Synthetasein Mouse Liver tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, blocked with PB ab64226 for 10 minutes at room temperature and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/200) for 30 minutes. A HRP-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"