Recombinant Anti-Glutamine Synthetase antibody [EPR16661] (ab197024)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16661] to Glutamine Synthetase
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Glutamine Synthetase antibody [EPR16661]
See all Glutamine Synthetase primary antibodies -
Description
Rabbit monoclonal [EPR16661] to Glutamine Synthetase -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human fetal liver, Human glioma, Mouse brain and Mouse spleen lysates; NIH/3T3 and HeLa cell lysates. IHC-P: Human hepatocellular carcinoma tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16661 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
KO cell pellets
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
WB |
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
|
Notes |
---|
IHC-P
1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
Target
-
Function
This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts. -
Involvement in disease
Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid. -
Sequence similarities
Belongs to the glutamine synthetase family. -
Developmental stage
Expressed during early fetal stages. -
Cellular localization
Cytoplasm. Mitochondrion. - Information by UniProt
-
Database links
- Entrez Gene: 2752 Human
- Entrez Gene: 14645 Mouse
- Omim: 138290 Human
- SwissProt: P15104 Human
- SwissProt: P15105 Mouse
- Unigene: 518525 Human
- Unigene: 210745 Mouse
-
Alternative names
- cell proliferation-inducing protein 59 antibody
- Cgl2214 antibody
- GLNA antibody
see all
Images
-
All lanes : Anti-Glutamine Synthetase antibody [EPR16661] (ab197024) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : GLUL knockout HeLa cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDaLanes 1- 2: Merged signal (red and green). Green - ab197024 observed at 42 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) observed at 50 kDa.
ab197024 was shown to react with Glutamine Synthetase in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261737 (knockout cell lysate ab256930) was used. Wild-type HeLa and GLUL knockout HeLa cell lysates were subjected to SDS-PAGE. ab197024 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Glutamine Synthetase knockout HAP1 whole cell lysate (20 µg)
Lane 3: Human brain whole cell lysate (20 µg)
Lane 4: A431 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab197024 observed at 42 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab197024 was shown to specifically react with Glutamine Synthetase in wild-type HAP1 cells as signal was lost in Glutamine Synthetase knockout cells. Wild-type and Glutamine Synthetase knockout samples were subjected to SDS-PAGE. ab197024 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging. -
All lanes : Anti-Glutamine Synthetase antibody [EPR16661] (ab197024) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
Anti-Glutamine Synthetase antibody [EPR16661] (ab197024) at 1/1000 dilution + Human glioma lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
-
All lanes : Anti-Glutamine Synthetase antibody [EPR16661] (ab197024) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse spleen lysate
Lane 3 : NIH/3T3 (Mouse embryo fibroblast cells) lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [EPR16661] (ab197024)
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Glutamine Synthetase with ab197024 at 1/8000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human hepatocellular carcinoma tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab197024 has not yet been referenced specifically in any publications.