Product nameAnti-Glutamyl Prolyl tRNA synthetase antibody
See all Glutamyl Prolyl tRNA synthetase primary antibodies
DescriptionRabbit polyclonal to Glutamyl Prolyl tRNA synthetase
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan
Synthetic peptide corresponding to a region between amino acids 950-1000 of Human Glutamyl Prolyl tRNA synthetase (NP_004437.2).
- 293T, HeLa, Jurkat, TCMK1 and NIH3T3 whole cell lysates
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab157122 is affinity purified using an epitope specific to Glutamyl Prolyl tRNA synthetase immobilized on solid support.
Our Abpromise guarantee covers the use of ab157122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 171 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionCatalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA.
Sequence similaritiesIn the N-terminal section; belongs to the class-I aminoacyl-tRNA synthetase family.
In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.
Contains 3 WHEP-TRS domains.
DomainThe WHEP-TRS domain is involved in RNA binding.
modificationsPhosphorylated at Ser-886 by CDK5 in a IFN-gamma-dependent manner; this phosphorylation activates assembly of the GAIT (IFN-gamma-activated inhibitor of translation) complex, which suppresses expression of a post-transcriptional regulon of proinflammatory genes in myeloid cells.
- Information by UniProt
- Bifunctional aminoacyl tRNA synthetase antibody
- Bifunctional aminoacyl-tRNA synthetase antibody
- Bifunctional glutamate/proline tRNA ligase antibody
All lanes : Anti-Glutamyl Prolyl tRNA synthetase antibody (ab157122) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 171 kDa
Exposure time: 10 seconds
Detection of Glutamyl Prolyl tRNA synthetase by Western Blot of Immunprecipitate.
ab157122 at 1 µg/ml labeling Glutamyl Prolyl tRNA synthetase in 293T whole cell lysate immunoprecipitated using ab157122 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds
ab157122 has not yet been referenced specifically in any publications.