Product nameAnti-Glutamyl Prolyl tRNA synthetase antibody
See all Glutamyl Prolyl tRNA synthetase primary antibodies
DescriptionRabbit polyclonal to Glutamyl Prolyl tRNA synthetase
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
- 293T, HeLa and Jurkat cells whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Note: pH 7 to 8.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab157129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 171 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionCatalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA.
Sequence similaritiesIn the N-terminal section; belongs to the class-I aminoacyl-tRNA synthetase family.
In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.
Contains 3 WHEP-TRS domains.
DomainThe WHEP-TRS domain is involved in RNA binding.
modificationsPhosphorylated at Ser-886 by CDK5 in a IFN-gamma-dependent manner; this phosphorylation activates assembly of the GAIT (IFN-gamma-activated inhibitor of translation) complex, which suppresses expression of a post-transcriptional regulon of proinflammatory genes in myeloid cells.
- Information by UniProt
- Bifunctional aminoacyl tRNA synthetase antibody
- Bifunctional aminoacyl-tRNA synthetase antibody
- Bifunctional glutamate/proline tRNA ligase antibody
All lanes : Anti-Glutamyl Prolyl tRNA synthetase antibody (ab157129) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 171 kDa
Exposure time: 10 seconds
Detection of Glutamyl Prolyl tRNA synthetase in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab157129 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.
ab157129 has not yet been referenced specifically in any publications.