Product nameAnti-Glutamyl Prolyl tRNA synthetase/PARS antibody
See all Glutamyl Prolyl tRNA synthetase/PARS primary antibodies
DescriptionRabbit polyclonal to Glutamyl Prolyl tRNA synthetase/PARS
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan
Synthetic peptide corresponding to Human Glutamyl Prolyl tRNA synthetase/PARS aa 950-1000.
Database link: NP_004437.2
- 293T, HeLa, Jurkat, TCMK1 and NIH3T3 whole cell lysates
This product was previously labelled as Glutamyl Prolyl tRNA synthetase
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab157122 is affinity purified using an epitope specific to Glutamyl Prolyl tRNA synthetase/PARS immobilized on solid support.
Our Abpromise guarantee covers the use of ab157122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 171 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionCatalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA.
Sequence similaritiesIn the N-terminal section; belongs to the class-I aminoacyl-tRNA synthetase family.
In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.
Contains 3 WHEP-TRS domains.
DomainThe WHEP-TRS domain is involved in RNA binding.
modificationsPhosphorylated at Ser-886 by CDK5 in a IFN-gamma-dependent manner; this phosphorylation activates assembly of the GAIT (IFN-gamma-activated inhibitor of translation) complex, which suppresses expression of a post-transcriptional regulon of proinflammatory genes in myeloid cells.
- Information by UniProt
- Bifunctional aminoacyl tRNA synthetase antibody
- Bifunctional aminoacyl-tRNA synthetase antibody
- Bifunctional glutamate/proline tRNA ligase antibody
All lanes : Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody (ab157122) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 171 kDa
Exposure time: 10 seconds
Detection of Glutamyl Prolyl tRNA synthetase/PARS by Western Blot of Immunprecipitate.
ab157122 at 1 µg/ml labeling Glutamyl Prolyl tRNA synthetase/PARS in 293T whole cell lysate immunoprecipitated using ab157122 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds
ab157122 has not yet been referenced specifically in any publications.