Overview

  • Product name
    Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody
    See all Glutamyl Prolyl tRNA synthetase/PARS primary antibodies
  • Description
    Rabbit polyclonal to Glutamyl Prolyl tRNA synthetase/PARS
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Glutamyl Prolyl tRNA synthetase/PARS aa 150-200.
    Database link: NP_004437.2

  • Positive control
    • 293T, HeLa and Jurkat whole cell lysates.
  • General notes

     This product was previously labelled as Glutamyl Prolyl tRNA synthetase

     

Properties

Applications

Our Abpromise guarantee covers the use of ab157128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 171 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA.
  • Sequence similarities
    In the N-terminal section; belongs to the class-I aminoacyl-tRNA synthetase family.
    In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.
    Contains 3 WHEP-TRS domains.
  • Domain
    The WHEP-TRS domain is involved in RNA binding.
  • Post-translational
    modifications
    Phosphorylated at Ser-886 by CDK5 in a IFN-gamma-dependent manner; this phosphorylation activates assembly of the GAIT (IFN-gamma-activated inhibitor of translation) complex, which suppresses expression of a post-transcriptional regulon of proinflammatory genes in myeloid cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bifunctional aminoacyl tRNA synthetase antibody
    • Bifunctional aminoacyl-tRNA synthetase antibody
    • Bifunctional glutamate/proline tRNA ligase antibody
    • Cell proliferation-inducing gene 32 protein antibody
    • DKFZp313B047 antibody
    • EARS antibody
    • Eprs antibody
    • GLNS antibody
    • Glu pro tRNA synthetase antibody
    • GLUPRORS antibody
    • GluRS antibody
    • Glutamate tRNA ligase antibody
    • Glutamatyl prolyl tRNA synthetase antibody
    • Glutaminyl tRNA synthetase antibody
    • Glutamyl prolyl tRNA synthetase antibody
    • Glutamyl tRNA synthetase antibody
    • Glutamyl-tRNA synthetase antibody
    • PARS antibody
    • PIG 32 antibody
    • PIG32 antibody
    • Proliferation inducing gene 32 protein antibody
    • Proliferation inducing protein 32 antibody
    • Proline tRNA ligase antibody
    • Proline--tRNA ligase antibody
    • Prolyl tRNA synthetase antibody
    • Prolyl-tRNA synthetase antibody
    • QARS antibody
    • QPRS antibody
    • SYEP_HUMAN antibody
    see all

Images

  • All lanes : Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody (ab157128) at 0.1 µg/ml

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 171 kDa


    Exposure time: 10 seconds
  • Detection of Glutamyl Prolyl tRNA synthetase/PARS by Western Blot of Immunprecipitate.
    ab157128 at 1µg/ml labeling Glutamyl Prolyl tRNA synthetase/PARS in 293T whole cell lysate immunoprecipitated using ab157128 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 3 seconds.

References

ab157128 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab157128.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up