Key features and details
- Rabbit polyclonal to Glutamyl Prolyl tRNA synthetase/PARS
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Glutamyl Prolyl tRNA synthetase/PARS antibody
See all Glutamyl Prolyl tRNA synthetase/PARS primary antibodies
DescriptionRabbit polyclonal to Glutamyl Prolyl tRNA synthetase/PARS
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan
Synthetic peptide corresponding to Human Glutamyl Prolyl tRNA synthetase/PARS aa 150-200.
Database link: NP_004437.2
- 293T, HeLa and Jurkat whole cell lysates.
This product was previously labelled as Glutamyl Prolyl tRNA synthetase
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab157128 was affinity purified using an epitope specific to Glutamyl Prolyl tRNA synthetase/PARS immobilized on solid support.
Our Abpromise guarantee covers the use of ab157128 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 171 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionCatalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA.
Sequence similaritiesIn the N-terminal section; belongs to the class-I aminoacyl-tRNA synthetase family.
In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.
Contains 3 WHEP-TRS domains.
DomainThe WHEP-TRS domain is involved in RNA binding.
modificationsPhosphorylated at Ser-886 by CDK5 in a IFN-gamma-dependent manner; this phosphorylation activates assembly of the GAIT (IFN-gamma-activated inhibitor of translation) complex, which suppresses expression of a post-transcriptional regulon of proinflammatory genes in myeloid cells.
- Information by UniProt
- Bifunctional aminoacyl tRNA synthetase antibody
- Bifunctional aminoacyl-tRNA synthetase antibody
- Bifunctional glutamate/proline tRNA ligase antibody
All lanes : Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody (ab157128) at 0.1 µg/ml
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 171 kDa
Exposure time: 10 seconds
Detection of Glutamyl Prolyl tRNA synthetase/PARS by Western Blot of Immunprecipitate.
ab157128 at 1µg/ml labeling Glutamyl Prolyl tRNA synthetase/PARS in 293T whole cell lysate immunoprecipitated using ab157128 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
ab157128 has not yet been referenced specifically in any publications.