Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody (ab31531)
- Datasheet
- References (12)
- Protocols
Overview
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Product nameAnti-Glutamyl Prolyl tRNA synthetase/PARS antibody
See all Glutamyl Prolyl tRNA synthetase/PARS primary antibodies -
DescriptionRabbit polyclonal to Glutamyl Prolyl tRNA synthetase/PARS
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Host speciesRabbit
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SpecificityThis antibody reacts specifically with EPRS protein.
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Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
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Species reactivityReacts with: Mouse, Human, Pig
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Immunogen
Recombinant full length protein corresponding to Human Glutamyl Prolyl tRNA synthetase/PARS.
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General notes
This product was previously labelled as Glutamyl Prolyl tRNA synthetase
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.05% Sodium azide
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PurityWhole antiserum
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab31531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 21460219 | |
| WB | 1/20000. Predicted molecular weight: 163 kDa. | |
| IP | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionCatalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA.
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Sequence similaritiesIn the N-terminal section; belongs to the class-I aminoacyl-tRNA synthetase family.
In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.
Contains 3 WHEP-TRS domains. -
DomainThe WHEP-TRS domain is involved in RNA binding.
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Post-translational
modificationsPhosphorylated at Ser-886 by CDK5 in a IFN-gamma-dependent manner; this phosphorylation activates assembly of the GAIT (IFN-gamma-activated inhibitor of translation) complex, which suppresses expression of a post-transcriptional regulon of proinflammatory genes in myeloid cells. - Information by UniProt
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Database links
- Entrez Gene: 2058 Human
- Entrez Gene: 107508 Mouse
- Entrez Gene: 449526 Pig
- Omim: 138295 Human
- SwissProt: P07814 Human
- SwissProt: Q8CGC7 Mouse
- Unigene: 497788 Human
- Unigene: 154511 Mouse
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Alternative names
- Bifunctional aminoacyl tRNA synthetase antibody
- Bifunctional aminoacyl-tRNA synthetase antibody
- Bifunctional glutamate/proline tRNA ligase antibody
see all
Images
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Western blot - Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody (ab31531)Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody (ab31531) at 1/10000 dilution + HEK293 cell line at 30 µg
Predicted band size: 163 kDa
Observed band size: 163 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody (ab31531)Ab31531 staining Glutamyl Prolyl tRNA synthetase/PARS in Hela cell line by Immunofluorescence.
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Western blot - Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody (ab31531)Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody (ab31531) at 1/20000 dilution + whole cell lysate prepared from HEK-293 cells at 50 µg/ml
Secondary
Goat anti-rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 163 kDa
Observed band size: 155 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody (ab31531)IHC image of ab31531 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31531, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunocytochemistry/ Immunofluorescence - Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody (ab31531)This image is courtesy of an anonymous Abreviewab31531 staining Glutamyl Prolyl tRNA synthetase/PARS in the Human Liver cell line Huh7 by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA, permeabilized with Triton X-100 0.5% and blocked with 10% Serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/300 in PBS + 10% Serum) for 1 hour at 25°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Garcia-Moreno M et al. System-wide Profiling of RNA-Binding Proteins Uncovers Key Regulators of Virus Infection. Mol Cell 74:196-211.e11 (2019). Read more (PubMed: 30799147) »
- Amorim IS et al. Loss of eIF4E Phosphorylation Engenders Depression-like Behaviors via Selective mRNA Translation. J Neurosci 38:2118-2133 (2018). Read more (PubMed: 29367404) »
