Key features and details
- Rabbit polyclonal to Glutamyl Prolyl tRNA synthetase/PARS - C-terminal
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Glutamyl Prolyl tRNA synthetase/PARS antibody - C-terminal
See all Glutamyl Prolyl tRNA synthetase/PARS primary antibodies
DescriptionRabbit polyclonal to Glutamyl Prolyl tRNA synthetase/PARS - C-terminal
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
Recombinant fragment within Human Glutamyl Prolyl tRNA synthetase/PARS (C terminal). The exact sequence is proprietary.
Database link: P07814
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extract. IHC-P: Mouse intestine tissue; Rat brain tissue.
This product was previously labelled as Glutamyl Prolyl tRNA synthetase
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 171 kDa.|
|IP||1/100 - 1/500.|
FunctionCatalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA.
Sequence similaritiesIn the N-terminal section; belongs to the class-I aminoacyl-tRNA synthetase family.
In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.
Contains 3 WHEP-TRS domains.
DomainThe WHEP-TRS domain is involved in RNA binding.
modificationsPhosphorylated at Ser-886 by CDK5 in a IFN-gamma-dependent manner; this phosphorylation activates assembly of the GAIT (IFN-gamma-activated inhibitor of translation) complex, which suppresses expression of a post-transcriptional regulon of proinflammatory genes in myeloid cells.
- Information by UniProt
- Bifunctional aminoacyl tRNA synthetase antibody
- Bifunctional aminoacyl-tRNA synthetase antibody
- Bifunctional glutamate/proline tRNA ligase antibody
All lanes : Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody - C-terminal (ab229498) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 171 kDa
5% SDS-PAGE gel.
Paraffin-embedded mouse intestine tissue stained for Glutamyl Prolyl tRNA synthetase/PARS using ab229498 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded rat brain tissue stained for Glutamyl Prolyl tRNA synthetase/PARS using ab229498 at 1/500 dilution in immunohistochemical analysis.
Glutamyl Prolyl tRNA synthetase/PARS was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg of ab229498. Western blot was performed from the immunoprecipitate using ab229498. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG instead of ab229498 in HEK-293T whole cell extract.
Lane 2: ab229498 IP in HEK-293T whole cell extract.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229498 has not yet been referenced specifically in any publications.