Product nameAnti-Glutamyl Prolyl tRNA synthetase/PARS antibody - C-terminal
See all Glutamyl Prolyl tRNA synthetase/PARS primary antibodies
DescriptionRabbit polyclonal to Glutamyl Prolyl tRNA synthetase/PARS - C-terminal
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
Recombinant fragment within Human Glutamyl Prolyl tRNA synthetase/PARS (C terminal). The exact sequence is proprietary.
Database link: P07814
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extract. IHC-P: Mouse intestine tissue; Rat brain tissue.
This product was previously labelled as Glutamyl Prolyl tRNA synthetase
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 171 kDa.|
|IP||1/100 - 1/500.|
FunctionCatalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA.
Sequence similaritiesIn the N-terminal section; belongs to the class-I aminoacyl-tRNA synthetase family.
In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.
Contains 3 WHEP-TRS domains.
DomainThe WHEP-TRS domain is involved in RNA binding.
modificationsPhosphorylated at Ser-886 by CDK5 in a IFN-gamma-dependent manner; this phosphorylation activates assembly of the GAIT (IFN-gamma-activated inhibitor of translation) complex, which suppresses expression of a post-transcriptional regulon of proinflammatory genes in myeloid cells.
- Information by UniProt
- Bifunctional aminoacyl tRNA synthetase antibody
- Bifunctional aminoacyl-tRNA synthetase antibody
- Bifunctional glutamate/proline tRNA ligase antibody
All lanes : Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody - C-terminal (ab229498) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 171 kDa
5% SDS-PAGE gel.
Paraffin-embedded mouse intestine tissue stained for Glutamyl Prolyl tRNA synthetase/PARS using ab229498 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded rat brain tissue stained for Glutamyl Prolyl tRNA synthetase/PARS using ab229498 at 1/500 dilution in immunohistochemical analysis.
Glutamyl Prolyl tRNA synthetase/PARS was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg of ab229498. Western blot was performed from the immunoprecipitate using ab229498. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG instead of ab229498 in HEK-293T whole cell extract.
Lane 2: ab229498 IP in HEK-293T whole cell extract.
ab229498 has not yet been referenced specifically in any publications.