Anti-Glutaredoxin 1 antibody (ab45953)
Key features and details
- Rabbit polyclonal to Glutaredoxin 1
- Suitable for: IHC-P, IP, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Glutaredoxin 1 antibody
See all Glutaredoxin 1 primary antibodies -
Description
Rabbit polyclonal to Glutaredoxin 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIP HumanWB Human
-
Immunogen
Synthetic peptide corresponding to Human Glutaredoxin 1 aa 50 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab45952) -
Positive control
- Recombinant Human Glutaredoxin 1 protein (ab86987) can be used as a positive control in WB. This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab45953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IP |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
Rabbit
Cow
Pig
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
| IP |
Use a concentration of 5 µg/ml.
|
|
| ICC/IF |
Use a concentration of 1 µg/ml.
|
|
| WB | (2) |
1/250. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
|
| Notes |
|---|
|
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
IP
Use a concentration of 5 µg/ml. |
|
ICC/IF
Use a concentration of 1 µg/ml. |
|
WB
1/250. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa). |
Target
-
Function
Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins. -
Sequence similarities
Belongs to the glutaredoxin family.
Contains 1 glutaredoxin domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 515416 Cow
- Entrez Gene: 2745 Human
- Entrez Gene: 93692 Mouse
- Entrez Gene: 397463 Pig
- Entrez Gene: 64045 Rat
- Omim: 600443 Human
- SwissProt: P10575 Cow
- SwissProt: P35754 Human
see all -
Alternative names
- GLRX antibody
- Glrx1 antibody
- GLRX1_HUMAN antibody
see all
Images
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Western blot - Anti-Glutaredoxin 1 antibody (ab45953)Anti-Glutaredoxin 1 antibody (ab45953) at 1/250 dilution + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 12 kDa
Observed band size: 12 kDa
Additional bands at: 45 kDa, 70 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-Glutaredoxin 1 antibody (ab45953)ICC/IF image of ab45953 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab45953, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaredoxin 1 antibody (ab45953)IHC image of ab45953 staining in cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45953, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-Glutaredoxin 1 antibody (ab45953) -
Immunoprecipitation - Anti-Glutaredoxin 1 antibody (ab45953)Glutaredoxin 1 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Glutaredoxin 1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab45953.
Secondary: Clean-Blot IP Detection Reagent (HRP) at 1/500 dilution.
Band: 12kDa; Glutaredoxin 1.
Protocols
References (13)
ab45953 has been referenced in 13 publications.
- You Y et al. Glutaredoxin 1 up-regulates deglutathionylation of a4 integrin and thereby restricts neutrophil mobilization from bone marrow. J Biol Chem 294:2616-2627 (2019). PubMed: 30598505
- Kommaddi RP et al. Glutaredoxin1 Diminishes Amyloid Beta-Mediated Oxidation of F-Actin and Reverses Cognitive Deficits in an Alzheimer's Disease Mouse Model. Antioxid Redox Signal 31:1321-1338 (2019). PubMed: 31617375
- Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
- Zlatic SA et al. Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees. Cell Syst 6:368-380.e6 (2018). PubMed: 29397366
- Zhu J et al. Oxidative and anti-oxidative status in muscle of young rats in response to six protein diets. Sci Rep 7:13184 (2017). PubMed: 29030561
