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Anti-Glutaredoxin 1 antibody (ab45953)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (13)

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Western blot - Anti-Glutaredoxin 1 antibody (ab45953)
  • Immunocytochemistry/ Immunofluorescence - Anti-Glutaredoxin 1 antibody (ab45953)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaredoxin 1 antibody (ab45953)
  • Western blot - Anti-Glutaredoxin 1 antibody (ab45953)
  • Immunoprecipitation - Anti-Glutaredoxin 1 antibody (ab45953)

Key features and details

  • Rabbit polyclonal to Glutaredoxin 1
  • Suitable for: IHC-P, IP, ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Glutaredoxin 1 antibody
    See all Glutaredoxin 1 primary antibodies
  • Description

    Rabbit polyclonal to Glutaredoxin 1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Glutaredoxin 1 aa 50 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab45952)

  • Positive control

    • Recombinant Human Glutaredoxin 1 protein (ab86987) can be used as a positive control in WB. This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant Human Glutaredoxin 1 protein (ab86987)
  • Recombinant Protein

    • Recombinant Human Glutaredoxin 1 protein (ab86987)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab45953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IP
Human
WB
Human
All applications
Mouse
Rat
Rabbit
Cow
Pig
Application Abreviews Notes
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP
Use a concentration of 5 µg/ml.
ICC/IF
Use a concentration of 1 µg/ml.
WB (2)
1/250. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
Notes
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP
Use a concentration of 5 µg/ml.
ICC/IF
Use a concentration of 1 µg/ml.
WB
1/250. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).

Target

  • Function

    Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.
  • Sequence similarities

    Belongs to the glutaredoxin family.
    Contains 1 glutaredoxin domain.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P35754 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 515416 Cow
    • Entrez Gene: 2745 Human
    • Entrez Gene: 93692 Mouse
    • Entrez Gene: 397463 Pig
    • Entrez Gene: 64045 Rat
    • Omim: 600443 Human
    • SwissProt: P10575 Cow
    • SwissProt: P35754 Human
    • SwissProt: Q9QUH0 Mouse
    • SwissProt: P12309 Pig
    • SwissProt: P12864 Rabbit
    • SwissProt: Q9ESH6 Rat
    • Unigene: 28988 Human
    • Unigene: 712696 Human
    • Unigene: 25844 Mouse
    • Unigene: 1484 Rat
    see all
  • Alternative names

    • GLRX antibody
    • Glrx1 antibody
    • GLRX1_HUMAN antibody
    • Glutaredoxin antibody
    • Glutaredoxin-1 antibody
    • Glutaredoxin1 antibody
    • Grx 1 antibody
    • Grx antibody
    • Grx1 antibody
    • MGC117407 antibody
    • Thioltransferase 1 antibody
    • Thioltransferase antibody
    • Thioltransferase-1 antibody
    • Thioltransferase1 antibody
    • TTase antibody
    • TTase 1 antibody
    • TTase-1 antibody
    • TTase1 antibody
    see all

Images

  • Western blot - Anti-Glutaredoxin 1 antibody (ab45953)
    Western blot - Anti-Glutaredoxin 1 antibody (ab45953)
    Anti-Glutaredoxin 1 antibody (ab45953) at 1/250 dilution + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 12 kDa
    Observed band size: 12 kDa
    Additional bands at: 45 kDa, 70 kDa. We are unsure as to the identity of these extra bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-Glutaredoxin 1 antibody (ab45953)
    Immunocytochemistry/ Immunofluorescence - Anti-Glutaredoxin 1 antibody (ab45953)
    ICC/IF image of ab45953 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab45953, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaredoxin 1 antibody (ab45953)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaredoxin 1 antibody (ab45953)
    IHC image of ab45953 staining in cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45953, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-Glutaredoxin 1 antibody (ab45953)
    Western blot - Anti-Glutaredoxin 1 antibody (ab45953)
  • Immunoprecipitation - Anti-Glutaredoxin 1 antibody (ab45953)
    Immunoprecipitation - Anti-Glutaredoxin 1 antibody (ab45953)
    Glutaredoxin 1 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Glutaredoxin 1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab45953.
    Secondary: Clean-Blot IP Detection Reagent (HRP) at 1/500 dilution.
    Band: 12kDa; Glutaredoxin 1.

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (13)

    Publishing research using ab45953? Please let us know so that we can cite the reference in this datasheet.

    ab45953 has been referenced in 13 publications.

    • You Y  et al. Glutaredoxin 1 up-regulates deglutathionylation of a4 integrin and thereby restricts neutrophil mobilization from bone marrow. J Biol Chem 294:2616-2627 (2019). PubMed: 30598505
    • Kommaddi RP  et al. Glutaredoxin1 Diminishes Amyloid Beta-Mediated Oxidation of F-Actin and Reverses Cognitive Deficits in an Alzheimer's Disease Mouse Model. Antioxid Redox Signal 31:1321-1338 (2019). PubMed: 31617375
    • Sikorski K  et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
    • Zlatic SA  et al. Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees. Cell Syst 6:368-380.e6 (2018). PubMed: 29397366
    • Zhu J  et al. Oxidative and anti-oxidative status in muscle of young rats in response to six protein diets. Sci Rep 7:13184 (2017). PubMed: 29030561
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-Glutaredoxin 1 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (rat heart)
    Gel Running Conditions
    Reduced Denaturing (15% resolving gel)
    Loading amount
    25 µg
    Specification
    rat heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 16 2016

    Western blot abreview for Anti-Glutaredoxin 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human Lens epithelial cells)
    Loading amount
    25 µg
    Specification
    Human Lens epithelial cells
    Gel Running Conditions
    Reduced Denaturing (12% SDS-PAGE)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 22 2011

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