Anti-Glutathione antibody (ab9443)


  • Product name
  • Description
    Rabbit polyclonal to Glutathione
  • Host species
  • Specificity
    Because these IgGs were specifically designed to detect glutaraldehyde-linked haptens rather than free GSH or GSSG, the only affinity data is based on IC50 values for artificial antigens constructed as J-GA-J reagents, where J = GSH or GSSG and GA = glutaraldehyde. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) was detected against L-alanine, gamma-aminobutyrate, D/L-aspartate, agmatine, guanidine, D/L-arginine, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. The antibody does not discriminate between free GSH and GSSG nor does it discriminate between oxidised and reduced GSH.It detects total "free" glutathione.
  • Tested applications
    Suitable for: IHC-FoFr, IHC-Fr, ICC, Immunomicroscopymore details
  • Immunogen

    Chemical/ Small Molecule by a Glutaraldehyde linker.



Our Abpromise guarantee covers the use of ab9443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration. This antibody is recommended for thin section post-embedding endogenous content mapping by Light and Electron microscopy immunostaining via High Performance Immunocytochemistry (HPI) (see "Protocols" tab above).


  • Relevance
    Glutathione is a small peptide composed of three amino acids: cysteine, glutamic acid, and glycine and is present in tissues in concentrations as high as one millimolar. It contains an unusual peptide linkage between the amine group of cysteine and the carboxyl group of the glutamate side chain. Glutathione is involved in detoxification, it binds to toxins, such as heavy metals, solvents, and pesticides, and transforms them into a form that can be excreted in urine or bile. It is also an important antioxidant, helping to maintain the -SH groups of proteins in their reduced form. Chronic functional glutathione deficiency is associated with glucose 6-phosphate dehydrogenase deficiency, immune disorders, an increased incidence of malignancies, and in the case of HIV disease, probably accelerated pathogenesis of the disease. Acute manifestations of functional glutathione deficiency can be seen in those who have taken an overdosage of acetaminophen (paracetamol). This results in depletion of glutathione in the hepatocytes, leading to liver failure and death.
  • Alternative names
    • Glutathione antibody
    • GSH antibody
    • Oxidised glutathione antibody
    • Reduced glutathione antibody


ab9443 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

I have been in touch with the lab regarding the concentration of this product, ab9443 anti-glutathione polyclonal. This IgG is prepared as an IgG fraction from serum. It is not an affinity purified pure anti-glutathione IgG so the lab does not have pro...

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ab9443 is a product designed for immunocytochemistry

1. ab9443 is an anti-glutathione polyclonal IgG prepared as an IgG fraction from serum. It is not an affinity purified pure anti-glutathione IgG. Thus it does not have protein concentr...

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"It is technically challenging and we do not recommend it for quantitative analysis.

The best method developed so far is that of Sun and Kalloniatis, J Comp Neurol 494:686 -703, 2006.

We advise to consider this...

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ab9443 is an anti-Glutathione polyclonal IgG prepared as an IgG fraction from serum. It is not an affinity purified pure anti-glutathione IgG thus we don't have a pure protein concentration for it.

As a very rough estimate, we believe that th...

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Thank you for your enquiry. The following publication shows glutathione (GSH) expression in frozen sections from various tissues including liver, lung, kidney, testis and eye (Fig. 3 & 4). HISTOCHEMICAL LOCALIZATION OF GLUTATHIONE IN TISSUES. T...

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Thank you for your enquiry. These are the references we have for ab9443: Joyce-Brady M, RE Marc, JC Jean 2002 The importance of gamma-Glutamyl transferase in lung glutathione homeostasis and antioxidant defense in Thiol Metabolism and Redox Regu...

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Thank you for your enquity. At the moment we have 2 glutathione antibodies, ab9443 and ab8883, neither of which have yet been tested for application in Western blotting. To see if another source may have what you are looking for, you can click on t...

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Yes. Antibodies against small molecule haptens (e.g. GTH) work in all species, including plants. As with all free small molecule targets, the fixative must include glutaraldehyde and the best visualization results obtained with silver intensification o...

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The antibody with which you are currently working (ab8883) has only been tested in ELISA. ab9443 has been tested in many IHC-related applications (Formalin fixed sections, Immunohistochemistry (Frozen Sections), Immunocytochemistry, Immunomicroscopy) s...

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