Product nameAnti-Glutathione antibody
See all Glutathione primary antibodies
DescriptionRabbit polyclonal to Glutathione
SpecificityBecause these IgGs were specifically designed to detect glutaraldehyde-linked haptens rather than free GSH or GSSG, the only affinity data is based on IC50 values for artificial antigens constructed as J-GA-J reagents, where J = GSH or GSSG and GA = glutaraldehyde. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) was detected against L-alanine, gamma-aminobutyrate, D/L-aspartate, agmatine, guanidine, D/L-arginine, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. The antibody does not discriminate between free GSH and GSSG nor does it discriminate between oxidised and reduced GSH.It detects total "free" glutathione.
Tested applicationsSuitable for: IHC-FoFr, IHC-Fr, ICC, Immunomicroscopymore details
Chemical/ Small Molecule by a Glutaraldehyde linker.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Thimerosal (merthiolate)
Constituents: PBS, Goat Serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab9443 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|Immunomicroscopy||Use at an assay dependent concentration. This antibody is recommended for thin section post-embedding endogenous content mapping by Light and Electron microscopy immunostaining via High Performance Immunocytochemistry (HPI) (see "Protocols" tab above).|
RelevanceGlutathione is a small peptide composed of three amino acids: cysteine, glutamic acid, and glycine and is present in tissues in concentrations as high as one millimolar. It contains an unusual peptide linkage between the amine group of cysteine and the carboxyl group of the glutamate side chain. Glutathione is involved in detoxification, it binds to toxins, such as heavy metals, solvents, and pesticides, and transforms them into a form that can be excreted in urine or bile. It is also an important antioxidant, helping to maintain the -SH groups of proteins in their reduced form. Chronic functional glutathione deficiency is associated with glucose 6-phosphate dehydrogenase deficiency, immune disorders, an increased incidence of malignancies, and in the case of HIV disease, probably accelerated pathogenesis of the disease. Acute manifestations of functional glutathione deficiency can be seen in those who have taken an overdosage of acetaminophen (paracetamol). This results in depletion of glutathione in the hepatocytes, leading to liver failure and death.
- Glutathione antibody
- GSH antibody
- Oxidised glutathione antibody
- Reduced glutathione antibody
ab9443 has not yet been referenced specifically in any publications.