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Thanks for your kindly suggestions, this customer indicated he already tried to modified his experiment so many times, including used 5% BSA as a blocking buffer, and also dilute the primary antibody to 1:250 and 1:150, however, the results still shows no band, he also indicated he already spend a lot of time to tried this experiment, therefore, he hopes we can help him to solve this problem as soon as possible.
Thanks for your kindly help
Asked on Oct 24 2012
Thank you for your message and for providing this further information.
I am sorry to hear the suggestions made have not improved the results on this occasion.
I appreciate the time your customer has spent on these experiments and would be pleased to arrange a replacement with a different lot number or a credit note in compensation.
I look forward to hearing from you with details of how you and your customer would like to proceed.
Answered on Oct 24 2012