Glutathione Detection Kit (Blue Fluorescence) (ab284552)
Key features and details
- Assay type: Cell-based
- Detection method: Fluorescent
- Platform: Microplate
Overview
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Product name
Glutathione Detection Kit (Blue Fluorescence)
See all Glutathione kits -
Detection method
Fluorescent -
Assay type
Cell-based -
Assay duration
Multiple steps standard assay -
Product overview
Glutathione (GSH) represents major low molecular-weight free thiol in living cells. As the key antioxidant in mammalian cells, intracellular GSH forms rapidly eliminated conjugates with electrophilic xenobiotics, free radicals as well as hydroperoxides.
Glutathione Detection Kit (ab284552) (previously known as EZCell Glutathione Detection Kit K504) utilizes Monochlorobimane (MCB), a non-fluorescent and cell-permeable dye, that forms highly fluorescent adducts with GSH (GSH-MCB) detectable at EX/EM= 380/465 ±20 nm respectively. This kit provides a useful tool for fast and easy evaluation of the GSH effectors directly in the living cells.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K504 EZCell™ Glutathione Detection Kit (Blue Fluorescence). K504-100 is the same size as the 100 test size of ab284552.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer 1 x 100ml Monochlorobimane (MCB) 1 x 1ml -
Research areas
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Relevance
Glutathione is a small peptide composed of three amino acids: cysteine, glutamic acid, and glycine and is present in tissues in concentrations as high as one millimolar. Glutathione is the principal intracellular low-molecular-weight thiol that plays a critical role in the cellular defense against oxidative and nitrosative stress in mammalian cells. Diminished glutathione levels have been observed in the early stages of apoptosis. -
Alternative names
- GSH
- GSSG
Images
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HeLa cells were seeded overnight at 5 x 105 of viable cells/well. The next day the cell culture was treated with 0.5 µg/ml of Staurosporine and returned to the incubator for the next 24 h. Levels of GSH in the living cells corresponding to the intensity of the signal were determined as described in the Assay Protocol. Fluorescence was measured directly in white opaque plates with clear bottoms used for cell culturing.
Comparison of GSH levels in control and apoptotic cells: Panel A: control, untreated cells with bright uniform GSH staining; Panel B: apoptotic cells with diminished blue fluorescence post 24 h treatment with Staurosporine. Dead cells are visualized by red staining of nuclear DNA with Propidium Iodide.
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HeLa cells were seeded overnight at 5 x 105 of viable cells/well. The next day the cell culture was treated with 0.5 µg/ml of Staurosporine and returned to the incubator for the next 24 h. Levels of GSH in the living cells corresponding to the intensity of the signal were determined as described in the Assay Protocol. Fluorescence was measured directly in white opaque plates with clear bottoms used for cell culturing.
GSH Fluorescence Curve of Jurkat cells prepared for this particular assay. Detection limit for corresponds to about 65,000 of Jurkat cells per well. Your results may not be identical to these. A new curve must be obtained for each experiment and the cell line.
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HeLa cells were seeded overnight at 5 x 105 of viable cells/well. The next day the cell culture was treated with 0.5 µg/ml of Staurosporine and returned to the incubator for the next 24 h. Levels of GSH in the living cells corresponding to the intensity of the signal were determined as described in the Assay Protocol. Fluorescence was measured directly in white opaque plates with clear bottoms used for cell culturing.
Reduced levels of fluorescence in apoptotic Jurkat cells. Decrease in RFU values between cells treated overnight with Staurosporine vs untreated positive control. Background values correspond to the cell culture without MCB treatment.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab284552 has not yet been referenced specifically in any publications.