Overview

  • Product name
    Anti-Glutathione Peroxidase 1 antibody
    See all Glutathione Peroxidase 1 primary antibodies
  • Description
    Sheep polyclonal to Glutathione Peroxidase 1
  • Host species
    Sheep
  • Tested applications
    Suitable for: Dot blot, IHC-P, IHC-Fr, WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    RRYSRRFQTIDIEPDIEALL

    , corresponding to C terminal amino acids 175-194 of Human Glutathione Peroxidase

  • General notes


    ab21966 reacts with cellular glutathione peroxidase. This is a 201 amino acid selenium dependant cytoplasmic enzyme that exists as a homotetramer. This enzyme protects red blood cells and other cells from oxidative damage.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab21966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/500000.

1/500,000 on 500 ng of antigen.

IHC-P 1/1000 - 1/20000.

Dilution depending on tissue and detection method. Antigen retrieval has been used in characterisation but may not be necessary.

IHC-Fr 1/1000 - 1/20000.

Dilution depending on tissue and detection method.

WB 1/10000 - 1/100000. Predicted molecular weight: 22 kDa.

Dilution depending on detection method.

ELISA 1/2000 - 1/20000.

Target

Images

  • DAB staining of microglia with GPx1S in human brain tissue. Primary antibody (1/400) secondary antibody donkey anti-sheep - biotin (1/3000) followed by Vector ABC kit.
  • DAB staining of microglia with GPx1S in human brain tissue. Primary antibody (1/1000) secondary antibody donkey anti-sheep - biotin (1/3000) followed by Vector ABC kit. (Oil emersion).

References

This product has been referenced in:
  • Kim IH  et al. Pre-treatment with Chrysanthemum indicum Linné extract protects pyramidal neurons from transient cerebral ischemia via increasing antioxidants in the gerbil hippocampal CA1 region. Mol Med Rep 16:133-142 (2017). IHC ; Gerbil . Read more (PubMed: 28534982) »
  • Seeher S  et al. Secisbp2 is essential for embryonic development and enhances selenoprotein expression. Antioxid Redox Signal 21:835-49 (2014). WB ; Mouse . Read more (PubMed: 24274065) »
See all 5 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Please check the online datasheets of all antibodies. You will find the required information.

Thanks!

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Answer

Thank you very much for contacting us.


The possible rabbit specific antibodies are available as;


ICAM1: the rabbit protein sequence (http://www.uniprot.org/uniprot/Q75T02) does not show any homology with human protein (http://www.uniprot.org/uniprot/P05362) so I am sorry we do not have any antibody available that can cross react with rabbit.


VCAM 1; ab98954 is the rabbit specific antibody.


SOD; ab13498


GPX: ab21966 is a good predicted candidate to try as the immunogen sequence is quite similar to rabbit (? http://www.uniprot.org/uniprot/P07203)


TNF alpha: ab66579 is predicted to work with rabbit as it has 90% immunogen sequence homology.


CRP: ab13426 immunogen sequence is homologous to rabbit so it is predicted to react.


Lipoxygenase; there are different forms of Lipoxygenase available e.g. LOX5, LOx12, LOX15. Which one you would interested in getting? ab23678 is anti LOX12 that can cross react with rabbit.

We do not have anti IL6 rabbit cross reactive antibody as the sequence of rabbit and human aren't homologous.


Where the antibodies are predicted to react with rabbit - you can avail advantage of testing discount programme. Please click the link for more information;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount


I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

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Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Treatment
Selenium poor or adequate feeding
Gel Running Conditions
Reduced Denaturing (Gel 12.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Aug 26 2009

Answer

Thank you for your enquiry and your patience. I apologize for the delay in obtaining the images, however, we now have some excellent images that we added to the online datasheet. This is a high titer antibody and when characterized on dot blots it will detect 500 ng of antigen at dilutions out to 1/500,000 which is much higher than most antibodies. A pathology laboratory has been very pleased with this antibody and the originator of the antibody has seen their immunohistochemical images at 1/30,000 which were still good. They have optimized their antigen retrieval techniques. We suggest using 1/1000 as a starting dilution, but you would need to characterize the antibody using your tissue and techniques. A particularly good feature of this antibody is that it has a very low background as seen in the images on the datasheet. I hope these images help you, and please contact us again if you have additional questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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