Key features and details
- Goat polyclonal to Glutathione Peroxidase 1
- Suitable for: IHC-Fr, IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-Glutathione Peroxidase 1 antibody
See all Glutathione Peroxidase 1 primary antibodies
DescriptionGoat polyclonal to Glutathione Peroxidase 1
SpecificityThis antibody is expected to recognise isoform 1 ( NP_000572.2) only.
Tested applicationsSuitable for: IHC-Fr, IHC-P, WBmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Guinea pig, Chimpanzee, Macaque monkey, Common marmoset
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab50427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 20 µg/ml.|
|IHC-P||Use a concentration of 3 - 5 µg/ml.|
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
1 hour primary incubation is recommended for this product.
FunctionProtects the hemoglobin in erythrocytes from oxidative breakdown.
Sequence similaritiesBelongs to the glutathione peroxidase family.
- Information by UniProt
- AL033363 antibody
- Cellular glutathione peroxidase antibody
- Glutathione peroxidase 1 antibody
Anti-Glutathione Peroxidase 1 antibody (ab50427) at 1 µg/ml + Human Liver lysate (35µg protein in RIPA buffer).
Predicted band size: 22 kDa
Observed band size: 22 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
ab50427 (20µg/ml) staining of PFA-perfused cryosection of Porcin Kidney following microwave antigen retrieval with citrate buffer pH 3 and CY3-staining shows staining of cytoplasm in PCT epithelial cells.
ab50437 at 3µg/ml staining Glutathione Perxidase 1 in human cerebral cortex by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by steam in citrate buffer pH 6.0. The AP staining procedure was used for staining.
ab50427 has been referenced in 2 publications.
- Ren Z et al. Sodium selenite inhibits deoxynivalenol-induced injury in GPX1-knockdown porcine splenic lymphocytes in culture. Sci Rep 8:17676 (2018). PubMed: 30518949
- Joyce NC et al. Age-related gene response of human corneal endothelium to oxidative stress and DNA damage. Invest Ophthalmol Vis Sci 52:1641-9 (2011). PubMed: 21087955