Western blot abreview for Anti-Glutathione Peroxidase 1 antibody

Average
Abreviews
Application
Western blot
Sample
Pig Tissue lysate - whole (Coronary arteries and arterioles)
Loading amount
20 µg
Specification
Coronary arteries and arterioles
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/25000

Detection

Detection method
Chemiluminescence
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 20 kDa Non-specific: 30 kDa

Dr. Wei Xie

Verified customer

Submitted Jul 28 2009

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