Western blot abreview for Anti-Glutathione Peroxidase 1 antibody

Excellent
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (LIVER)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
LIVER
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Incubation time
14 hour(s) and 0 minute(s)
Dilution
1/2000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: goat anti-rabbit antibody (Santa Cruz)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 22 kDa

Additional data

Additional Notes
Liver tissue were homogenized with RIPA buffer (RIPA buffer pH 8.6 containing 150 mM NaCI, 1% NP — 40, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, 5 mM EDTA, 1 mM EGTA, 10 mM sodium fluoride, 20 mM DTT, 1 mM benzamidine HCI, 1mMPMSF, 1 µM aprotinin, pepstatin A and okadaic acid, All lanes are loaded with rat liver tissue lysates from different high fat diet treatment groups Nitrocellulose membrane and CL – Xposure films were from Amersham ECL: WestPico Super Signal

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Submitted Oct 14 2016

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