Overview

  • Product name

    Anti-Glutathione Peroxidase 1 antibody - C-terminal
    See all Glutathione Peroxidase 1 primary antibodies
  • Description

    Rabbit polyclonal to Glutathione Peroxidase 1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Glutathione Peroxidase 1 aa 164-193 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P07203

  • Positive control

    • IHC-P: Human liver tissue. ICC/IF: HepG2 cells. WB: THP-1 cell lysate; Mouse and rat liver lysates. Flow Cyt: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab230882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.
IHC-P 1/100.
WB 1/1000. Predicted molecular weight: 22 kDa.
ICC/IF 1/10 - 1/50.

Target

Images

  • All lanes : Anti-Glutathione Peroxidase 1 antibody - C-terminal (ab230882) at 1/1000 dilution

    Lane 1 : THP-1 (Human monocytic leukemia cell line) cell lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : Rat liver tissue lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG H&L (HRP) at 1/5000 dilution

    Predicted band size: 22 kDa

  • HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for Glutathione Peroxidase 1 using ab230882 at 1/10 dilution in ICC/IF, followed by Alexa Fluor®488-conjugated Goat Anti-Rabbit IgG (green). DAPI was used to stain the cell nuclear (blue).

  • Formalin-fixed, paraffin-embedded human liver tissue stained for Glutathione Peroxidase 1 using ab230882 at 1/100 dilution in immunohistochemical analysis.

  • Flow cytometric analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, labeling Glutathione Peroxidase 1 with ab230882 at 1/10 dilution (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab230882 has not yet been referenced specifically in any publications.

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