Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)

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Overview

  • Product name

    Anti-Glutathione Peroxidase 1 antibody [EPR3311]
    See all Glutathione Peroxidase 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3311] to Glutathione Peroxidase 1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide. within Human Glutathione Peroxidase 1 aa 1-100 (internal sequence). The exact sequence is proprietary.
    Database link: P07203

  • Positive control

    • Human fetal liver, SH SY5Y, and THP1 cell lysates; Human breast carcinoma tissue.

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
ICC 1/50 - 1/100.
Flow Cyt Use at an assay dependent concentration.

Target

Images

  • Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)
    Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: GPX1 knockout HAP1 cell lysate (20 µg)
    Lane 3: THP1 cell lysate (20 µg)
    Lane 4: HL60 cell lysate (20 µg)
    Lanes 1 and 2: Merged signal (red and green). Green - ab108429, observed at 22 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab108429 was shown to specifically react with Glutathione Peroxidase 1 in wild-type HAP1 cells. No band was observed when Glutathione Peroxidase 1 knockout samples were examined. Wild-type and Glutathione Peroxidase 1 knockout samples were subjected to SDS-PAGE. ab108429 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)
    Flow Cytometry - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)

    Flow Cytometry analysis of THP-1 (human acute monocytic leukemia ) cells labeling Glutathione Peroxidase 1 with purified ab108429 at 1:250 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1:2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)
    Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)
    All lanes : Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : SH SY5Y cell lysate
    Lane 3 : THP1 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 22 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)

    ab108429, at 1/100 dilution, staining Glutathione Peroxidase 1 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Meimaridou E  et al. NNT is a key regulator of adrenal redox homeostasis and steroidogenesis in male mice. J Endocrinol 236:13-28 (2018). Read more (PubMed: 29046340) »
  • Sonet J  et al. Comparison of analytical methods using enzymatic activity, immunoaffinity and selenium-specific mass spectrometric detection for the quantitation of glutathione peroxidase 1. Anal Chim Acta 1011:11-19 (2018). Read more (PubMed: 29475480) »
See all 5 Publications for this product

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