Recombinant Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3311] to Glutathione Peroxidase 1
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Glutathione Peroxidase 1 antibody [EPR3311]
See all Glutathione Peroxidase 1 primary antibodies -
Description
Rabbit monoclonal [EPR3311] to Glutathione Peroxidase 1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver, SH SY5Y, and THP1 cell lysates; Human breast carcinoma tissue. WB: HEK-293T cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3311 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB |
1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
Target
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Function
Protects the hemoglobin in erythrocytes from oxidative breakdown. -
Sequence similarities
Belongs to the glutathione peroxidase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 2876 Human
- Entrez Gene: 24404 Rat
- Omim: 138320 Human
- SwissProt: P07203 Human
- SwissProt: P04041 Rat
- Unigene: 76686 Human
- Unigene: 11323 Rat
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Alternative names
- AL033363 antibody
- Cellular glutathione peroxidase antibody
- Glutathione peroxidase 1 antibody
see all
Images
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All lanes : Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : GPX1 knockout HEK-293T cell lysate
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDaFalse colour image of Western blot: Anti-Glutathione Peroxidase 1 antibody [EPR3311] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab108429 was shown to bind specifically to Glutathione Peroxidase 1. A band was observed at 22 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in GPX1 knockout cell line ab266650 (knockout cell lysate ab256932). To generate this image, wild-type and GPX1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: GPX1 knockout HAP1 cell lysate (20 µg)
Lane 3: THP1 cell lysate (20 µg)
Lane 4: HL60 cell lysate (20 µg)
Lanes 1 and 2: Merged signal (red and green). Green - ab108429, observed at 22 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab108429 was shown to specifically react with Glutathione Peroxidase 1 in wild-type HAP1 cells. No band was observed when Glutathione Peroxidase 1 knockout samples were examined. Wild-type and Glutathione Peroxidase 1 knockout samples were subjected to SDS-PAGE. ab108429 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
Intracellular Flow Cytometry analysis of THP-1 (human acute monocytic leukemia) cells labeling Glutathione Peroxidase 1 with purified ab108429 at 1/250 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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All lanes : Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : SH SY5Y cell lysate
Lane 3 : THP1 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 22 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)
ab108429, at 1/100 dilution, staining Glutathione Peroxidase 1 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab108429 has been referenced in 8 publications.
- Vindry C et al. A Versatile Strategy to Reduce UGA-Selenocysteine Recoding Efficiency of the Ribosome Using CRISPR-Cas9-Viral-Like-Particles Targeting Selenocysteine-tRNA[Ser]Sec Gene. Cells 8:N/A (2019). PubMed: 31212706
- Sonet J et al. Selenized Plant Oil Is an Efficient Source of Selenium for Selenoprotein Biosynthesis in Human Cell Lines. Nutrients 11:N/A (2019). PubMed: 31277500
- Jin L et al. The PLAG1-GDH1 Axis Promotes Anoikis Resistance and Tumor Metastasis through CamKK2-AMPK Signaling in LKB1-Deficient Lung Cancer. Mol Cell 69:87-99.e7 (2018). PubMed: 29249655
- Meimaridou E et al. NNT is a key regulator of adrenal redox homeostasis and steroidogenesis in male mice. J Endocrinol 236:13-28 (2018). PubMed: 29046340
- Sonet J et al. Comparison of analytical methods using enzymatic activity, immunoaffinity and selenium-specific mass spectrometric detection for the quantitation of glutathione peroxidase 1. Anal Chim Acta 1011:11-19 (2018). PubMed: 29475480
- Touat-Hamici Z et al. Selenium-regulated hierarchy of human selenoproteome in cancerous and immortalized cells lines. Biochim Biophys Acta N/A:N/A (2018). Human . PubMed: 29660373
- Chaisiriwong L et al. A Case-Control Study of Involvement of Oxidative DNA Damage and Alteration of Antioxidant Defense System in Patients with Basal Cell Carcinoma: Modulation by Tumor Removal. Oxid Med Cell Longev 2016:5934024 (2016). IHC . PubMed: 27057281
- Baqader NO et al. Nuclear cytoplasmic trafficking of proteins is a major response of human fibroblasts to oxidative stress. J Proteome Res 13:4398-423 (2014). WB ; Human . PubMed: 25133973